Nacalai USA, Inc. | Product | Column Selection Guide

Column Selection Guide

• Organic Compounds (Low M.W.)

Octadecyl group bonded column (C₁₈, ODS) are recommended as first-choice columns for separations of organic compounds (Low M.W.). If there is not enough separation or no retention using COSMOSIL C₁₈ columns, COSMOSIL series offer many kinds of special columns.

• Saccharides

COSMOSIL Sugar-D is recommended for the separation of monosaccharides and oligosaccharides as a first-choice column. For the separation of sugar derivatives, COSMOSIL C₁₈-PAQ is suitable as well.

• Proteins

Please select based on the separation mode.

• Fullerenes

COSMOSIL Buckyprep is most suitable for the separation of fullerenes.

Reversed Phase Specialty Columns

Reversed phase HPLC columns have been widely used because of their superior resolution, high theoretical plate number and ease of use. Since hydrophobic interaction is the dominant separation mechanism in reversed phase chromatography, conventional stationary phases such as C₁₈ and C₁₈ do not offer optimum selectivity for compounds with similar hydrophobicity. COSMOSIL offers a broad selection of columns with unique stationary phases for separation of these difficult analytes. These columns offer improved separation of structurally similar compounds that are difficult to analyze with a C₁₈ type column.

Specifications

Packing Material	Cholester			PBr		PFP	πNAP		PYE	NPE
Silica Gel	Fully-Porous		Core-Shell	Fully-Porous	Core-Shell	Fully-Porous	Fully-Porous		Fully-Porous	Fully-Porous
Particle Size	2.5 µm	5 µm	2.6 µm	5 µm	2.6 µm	5 µm	2.5 µm	5 µm	5 µm	5 µm
Pore Size	130Å	120Å	90Å	120Å	90Å	120Å	130Å	120Å	120Å	120Å
Specific Surface Area	300 m²/g	300 m²/g	150 m²/g	300 m²/g	150 m²/g	300 m²/g	300 m²/g	300 m²/g	300 m²/g	300 m²/g
Stationary Phase	Cholesteryl Group			Pentabromobenzyl group		Pentafluorophrnyl group	Naphthylethyl Group		Pyrenylethyl Group	Nitrophenylethyl Group
Bonding Type	Monomeric
Main Interaction	- Hydrophobic Interaction - Molecular Shape Selectivity			- Hydrophobic interaction - Dispersion force		- Hydrophobic interaction - π-π Interaction - Dipole-dipole Interaction	- Hydrophobic Interaction - π-π Interaction		- Hydrophobic Interaction - π-π Interaction - Dispersion Force - Molecular shape selectivity	- Hydrophobic Interaction - π-π Interaction - Dipole-dipole Interaction
End Capping Treatment	Near-perfect treatment
Carbon Content	21%	20%	-	8%	-	10%	14%	11%	18%	9%
pH Range	2–7.5
Feature	- Usable under the same conditions as C₁₈ - High molecular shape selectivity			- Separate hydrophilic compounds in reversed phase conditions - Separate using dispersion force		- Weak dipole-dipole interaction	- Stronger π-π interaction than phenyl column		- Very strong π-π interaction	- Strong dipole-dipole interaction

Fully-Porous: High purity porous spherical silica / Core-Shell: Core-Shell silica gel

Selectivity for positional isomers of dinitrobenzene

Different stationary phase exhibits different selectivity due to the use of forces that C18 (hydrophobic interaction) does not have. By using these columns, you can achieve separation that cannot be done using only C18.

COSMOSIL Column List by Stationary Phase

Columns for USP Specifications

COSMOSIL offers a comprehensive range of HPLC columns which meet the USP (United States Pharmacopoeia) specifications.

USP Code	Description	Recommended Columns
L1	Octadecyl silane chemically bonded to porous silica or ceramic micro-particles, 1.7 to 10 m in diameter, or a monolithic rod	COSMOSIL 3C₁₈EB COSMOSIL C₁₈-MS-II COSMOSIL C₁₈-AR-II COSMOSIL C₁₈-PAQ COSMOSIL C₁₈-AR-300 COSMOSIL Protein-R
L3	Porous silica particles, 5 to 10 µm in diameter	COSMOSIL SL-II
L7	Octylsilane chemically bonded to totally porous silica particles, 1.7 to 10 µm in diameter	COSMOSIL C₈-MS COSMOSIL C₈-AR-300
L8	An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 1.5 to 10 µm in diameter, or a monolithic silica rod.	COSMOSIL NH₂-MS
L10	Nitrile groups chemically bonded to porous silica particles, 3 to 10 µm in diameter	COSMOSIL CN-MS
L11	Phenyl groups chemically bonded to porous silica particles, 1.7 to 10 µm in diameter	COSMOSIL PE-MS COSMOSIL Ph-AR-300 COSMOSIL πNAP
L13	Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in diamete	COSMOSIL TMS-MS
L20	Dihydroxypropane groups chemically bonded to porous silica particles, 5 to 10 µm in diameter	COSMOSIL Diol-120-II COSMOSIL Diol-300-II COSMOSIL Diol-1000-II
L26	Butyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter	COSMOSIL C₄-MS COSMOSIL C₄-AR-300
L43	Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 1.5 to 10 µm in diameter	COSMOSIL PFP
L99	Amylose tris-(3,5-dumethylphenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 µm in diameter	COSMOSIL CHiRAL A
L101	Cholesteryl groups chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 µm in diameter, or a monolithic rod.	COSMOSIL Cholester COSMOCORE Cholester
L104	Triazole groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter	COSMOSIL HILIC
L119	Cellulose tris-(3,5-dichlorophenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 µm in diameter	COSMOSIL CHiRAL C

COSMOSIL Application Search

COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size

Column Selection Guide by Sample Type

Conventional Columns versus High Performance Columns

A period of more than 40 years has passed since the first COSMOSIL 5C18 columns were developed and offered for sale. Continuous technical improvement has made many of these columns obsolete and not of the highest quality and performance available any more. However, many long-term users continue to employ these older conventional columns for routine analysis and quality control. Nevertheless, the manufacture of these older columns will eventually cease and we strongly urge customers to replace the conventional columns with their higher performance equivalents outlined in the table below. For additional information, please contact us.

Conventional Columns (Old)		High Performance Columns (New)
5C18-AR	→	5C18-AR-II
5C18	→	5C18-MS-II
5C18-MS	→	5C18-MS-II
5C18-P	→	5C18-PAQ
5NH2	→	5NH2-MS

>> Click here for ordering information of Conventional Columns (old).