COSMOSIL PBr
Separation of hydrophilic compounds in reversed-phase conditions
- Separate hydrophilic compounds in reversed-phase conditions
- Simple mobile phase condition compared to HILIC
- More sample loading capacity than HILIC
- Alternative selectivity to C18 column
| COSMOSIL PBr | ||
|---|---|---|
| Silica gel | High purity porous spherical silica | |
| Average particle size | 3 µm | 5 µm |
| Average pore size | approx. 120Å | |
| Specific surface area | approx. 300 m²/g | |
| Stationary phase | Pentabromobenzyl group | |
| Bonding type | Monomeric | |
| Endcapping treatment | Yes | |
| Carbon content | - | approx. 8% |
| Usable pH range | 2 - 7.5 | |
Separation of hydrophilic compounds in reversed-phase conditions
Hydrophilic interaction chromatography (HILIC) is an increasingly popular analytical technique. However, it is often difficult to develop a robust method due to users' less familiarity with HILIC conditions. Furthermore, high concentration of acetonitrile used in HILIC mobile phase makes it extremely sensitive to samples' water concentration; injecting samples in high water concentration often results in poor peak shapes. COSMOSIL PBr enables separation of hydrophilic compounds in reversed phase conditions, maintaining sharp peak shapes even with aqueous samples.
| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | HILIC; Acetonitrile : Water = 90 : 10 / PBr; Water |
| Flow rate: | 1.0 ml/min |
| Temperature: | 30°C |
| Detection: | UV 260 nm |
| Sample: | 1. Uracil (0.1 mg/ml) 2. Uridine (0.2 mg/ml) |
| Sample solution: | Water |
Comparison with C18 Columns
COSMOSIL PBr retains hydrophilic compounds stronger than C18 columns under the same reversed-phase conditions.
| Column size: | 4.6 mmI.D. x 250 mm | |
| Mobile phase: | 20 mmol/l Phosphate Buffer (pH7.0) | |
| Flow rate: | 1.0 ml/min | |
| Temperature: | 30°C | |
| Detection: | UV 225 nm | |
| Sample: | 1. Ammelide (0.1 mg/ml) 2. Cyanuric Acid (0.5 mg/ml) 3. Ammeline (0.1 mg/ml) 4. Malamine (0.1 mg/ml) |
|
| Injection vol.: | 1.0 µl |
Separation Mechanism
In reversed-phase chromatography, compounds are separated by difference in hydrophobicity (the number of CH2 bases, see chromatogram on the left (1)). Compounds with little hydrophobicity (the number of OCH2CH2, see chromatogram on the right (2)), are not retained by a C18 column. However, these compounds can easily be separated by dispersion force interaction using the COSMOSIL PBr column.
Compounds with more hydrophobicity
| Column size: | 4.6 mmI.D. x 150 mm |  |
| Mobile phase: | Methanol : Water = 80 : 20 | |
| Flow rate: | 1.0 ml/min | |
| Temperature: | 30°C | |
| Detection: | UV 254 nm | |
| Sample: | 1. Benezene 2. Toluene 3. Ethylbenzene 4. n-Propylbenzene 5. n-Butylbenzene |
Compounds with less hydrophobicity
| Column size: | 4.6 mmI.D. x 150 mm |  |
| Mobile phase: | Methanol : Water = 60 : 40 | |
| Flow rate: | 1.0 ml/min | |
| Temperature: | 30°C | |
| Detection: | UV 254 nm | |
| Sample: | 1. Benzyl Alcohol 2. Ethylene Glycol Monobenzyl Ether 3. Diethylene Glycol Monobenzyl Ether 4. Triethylene Glycol Monobenzyl Ether 5. Tetraethylene Glycol Monobenzyl Ether |
Dispersion force (instantaneous dipole-induced dipole force)
London Dispersion force is a weak intermolecular force that results from dipoles temporarily induced from random unsymmetrical electron positions in two adjacent atoms, also known as "instantaneous dipole-induced dipole force". It is present in all molecules regardless if they are polar or non-polar. Compounds with high polarizability have stronger dispersion force.
Compounds with stronger dispersion force
- Larger and heavier molecules
- Molecules with larger and heavier atoms (e.g. from weakest to strongest in halogens, F2, Cl2, Br2, and I2)
- Molecules with delocalized electrons and resonance (e.g. aromatic compounds)
⇒ COSMOSIL PBr column is packed with pentabromobenzyl bonded silica that enables separation by dispersion force interaction.
Suitable Compounds to Analyze by COSMOSIL PBr
COSMOSIL PBr retains cyclic compounds or amide compounds more strongly than C18 columns.
(Mobile phase)
(1) Methanol : 20 mmol/l Phosphate buffer(pH7.0) = 10 : 90)
(2) 20 mmol/l Phosphate buffer (pH2.5))
(3) 20 mmol/l Phosphate buffer (pH7.0))
(4) Methanol : Water = 10 : 90
Column Stability Test
COSMOSIL PBr column remains stable after 1000 injections under 100% aqueous condition.
| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | 20 mmol/l Phosphate buffer (pH2.5) |
| Flow rate: | 1.0 ml/min |
| Temperature: | 40°C |
| Detection: | UV 280 nm |
| Sample: | 1. L-Noradrenaline (0.5 mg/ml) 2. L-Adrenaline (0.5 mg/ml) 3. Dopamine (0.5 mg/ml) 4. L-DOPA (0.5 mg/ml) |
| Injection vol.: | 1.0 µl |
Application
Oligonucleotides
Polyethylene Glycol (SFC)
.PNG)
Gentamicin
| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | 0.1% Pentafluoropropionic Acid –Acetonitrile : Water = 10 : 90 |
| Flow rate: | 0.2 ml/min |
| Temperature: | 40°C |
| Detection: | ESI-MS, Positive, SIM |
| Sample: | Gentamicin (C1a, C2, C2a, C1) |
| Injection vol.: | 1.0 µl |
Nucleic Acid Base
| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | Methanol : 20 mmol/l Phosphate buffer(pH7.0) = 10 : 90 |
| Flow rate: | 1.0 ml/min |
| Temperature: | 30°C |
| Detection: | UV 260 nm |
| Sample: | 1. Cytosine (0.05 mg/ml) 2. Uracil (0.05 mg/ml) 3. Thymine (0.05 mg/ml) 4. Guanine (0.05 mg/ml) 5. Adenine (0.05 mg/ml) |
| Injection vol.: | 1.0 µl |
Coenzyme A
| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | Methanol : 20 mmol/l Phosphate buffer(pH7.0) = 20 : 80 |
| Flow rate: | 1.0 ml/min |
| Temperature: | 30°C |
| Detection: | UV 260 nm |
| Sample: | 1. Malonyl Coenzyme A (0.25 mg/ml) 2. Coenzyme A (0.25 mg/ml) 3. Acetyl Coenzyme A (0.25 mg/ml) |
| Injection vol.: | 2.0 µl |
Methylimidazole Isomers
| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | 20 mmol/l Phosphate buffer(pH2.5) |
| Flow rate: | 1.0 ml/min |
| Temperature: | 30°C |
| Detection: | UV 220 nm |
| Sample: | 1. 1-Methylimidazole (0.1 mg/ml) 2. 2-Methylimidazole (0.1 mg/ml) 3. 4-Methylimidazole (0.1 mg/ml) |
| Injection vol.: | 1.0 µl |
Surfactants
COSMOSIL PBr shows excellent separation for surfactants compared to C18 or PFP columns.
| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | 5C18-MS-II, 5PFP; Methanol : Water = 80 : 20 PBr; Methanol |
| Flow rate: | 1.0 ml/min |
| Temperature: | 30°C |
| Detection: | UV 254 nm |
| Sample: | Triton® X-100 (50 mg/ml) |
| Injection vol.: | 2.0 µl |
Halogen Compounds
| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | Methanol : Water = 65 : 35 |
| Flow rate: | 1.0 ml/min |
| Temperature: | 30°C |
| Detection: | UV 254 nm |
| Sample: | 1. Fluorobenzene (1.0 mg/ml) 2. α,α,α-Trifluorotoluene [Benzotrifluoride] (0.25 mg/ml) 3. Toluene (2.5 mg/ml) 4. Chlorobenzene (5.0 mg/ml) 5. Bromobenzene (5.0 mg/ml) 6. Iodobenzene (2.0 mg/ml) |
| Injection vol.: | 1.0 µl |
Usage Information
| Column | 5 µm PBr | |||||
|---|---|---|---|---|---|---|
| I.D. (mm) | 2.0 | 3.0 | 4.6 | 10.0 | 20.0 | 28.0 |
| Washing method | 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. 2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF. |
|||||
| Storage conditions | Short-term (up to a week): Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. Long-term: Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water. In either case, tightly plug the column, and store in a cool and dry place. |
|||||
| Usable pH range | pH2～pH7.5 | |||||
| Max. pressure | 20MPa | 15MPa | ||||
| Temperature range | The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C. | |||||
| Usable solvents | Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable. Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points. |
|||||
| Other instructions | - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L. - Always filter mobile phases using a 0.5 µm or finer filter before use. - Acetonitrile is not recommended as a mobile phase. |
|||||
COSMOSIL Application Search
COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size
New Application: Lorazepam and Its Impurities A-E
Downloads / References
|
Brochures
|
COSMOSIL PBr
|
|
|
|
Polar molecule separation by COSMOSIL PBr
|
|
|
Posters
|
Evaluation of Pentabromobenzyl Stationary Phase for Separation of Polar Compounds by Reversed-Phase Chromatography
(Poster from HPLC 2015, Geneva) |
|
|
|
Fat-Soluble and Water-Soluble Vitamins in Functional Water
(Poster from Food Science Asia 2015, Singapore) |
|
| References |
|
|
(1.4 MB) Ordering Information
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL 3PBr Packed Column (2.0 mmI.D. x 50 mm ) | End-Fitting: Waters type |
19345-91 | 1 pkg | 664.00 | Buy |
| COSMOSIL 3PBr Packed Column (2.0 mmI.D. x 75 mm ) | End-Fitting: Waters type |
19346-81 | 1 pkg | 664.00 | Buy |
| COSMOSIL 3PBr Packed Column (2.0 mmI.D. x 100 mm ) | End-Fitting: Waters type |
19347-71 | 1 pkg | 664.00 | Buy |
| COSMOSIL 3PBr Packed Column (2.0 mmI.D. x 150 mm ) | End-Fitting: Waters type |
19078-01 | 1 pkg | 664.00 | Buy |
| COSMOSIL 3PBr Packed Column (2.0 mmI.D. x 250 mm ) | End-Fitting: Waters type |
19348-61 | 1 pkg | 792.00 | Buy |
| COSMOSIL 3PBr Packed Column (3.0 mmI.D. x 50 mm ) | End-Fitting: Waters type |
19349-51 | 1 pkg | 664.00 | Buy |
| COSMOSIL 3PBr Packed Column (3.0 mmI.D. x 75 mm ) | End-Fitting: Waters type |
19350-11 | 1 pkg | 664.00 | Buy |
| COSMOSIL 3PBr Packed Column (3.0 mmI.D. x 100 mm ) | End-Fitting: Waters type |
19351-01 | 1 pkg | 664.00 | Buy |
| COSMOSIL 3PBr Packed Column (3.0 mmI.D. x 150 mm ) | End-Fitting: Waters type |
19352-91 | 1 pkg | 664.00 | Buy |
| COSMOSIL 3PBr Packed Column (3.0 mmI.D. x 250 mm ) | End-Fitting: Waters type |
19353-81 | 1 pkg | 792.00 | Buy |
| COSMOSIL 3PBr Packed Column (4.6 mmI.D. x 50 mm ) | End-Fitting: Waters type |
19354-71 | 1 pkg | 664.00 | Buy |
| COSMOSIL 3PBr Packed Column (4.6 mmI.D. x 75 mm ) | End-Fitting: Waters type |
19355-61 | 1 pkg | 664.00 | Buy |
| COSMOSIL 3PBr Packed Column (4.6 mmI.D. x 100 mm) | End-Fitting: Waters type |
19356-51 | 1 pkg | 632.00 | Buy |
| COSMOSIL 3PBr Packed Column (4.6 mmI.D. x 150 mm) | End-Fitting: Waters type |
19357-41 | 1 pkg | 632.00 | Buy |
| COSMOSIL 3PBr Packed Column (4.6 mmI.D. x 250 mm) | End-Fitting: Waters type |
19358-31 | 1 pkg | 739.00 | Buy |
| COSMOSIL 5PBr Packed Column (1.0 mmI.D. x 150 mm) | End-Fitting: Waters type |
12881-71 | 1 pkg | 749.00 | Buy |
| COSMOSIL 5PBr Packed Column (2.0 mmI.D. x 50 mm) | End-Fitting: Waters type |
12943-61 | 1 pkg | 509.00 | Buy |
| COSMOSIL 5PBr Packed Column (2.0 mmI.D. x 100 mm) | End-Fitting: Waters type |
13245-81 | 1 pkg | 535.00 | Buy |
| COSMOSIL 5PBr Packed Column (2.0 mmI.D. x 150 mm) | End-Fitting: Waters type |
12392-81 | 1 pkg | 546.00 | Buy |
| COSMOSIL 5PBr Packed Column (2.0 mmI.D. x 250 mm) | End-Fitting: Waters type |
13247-61 | 1 pkg | 659.00 | Buy |
| COSMOSIL 5PBr Packed Column (3.0 mmI.D. x 50 mm) | End-Fitting: Waters type |
12592-61 | 1 pkg | 573.00 | Buy |
| COSMOSIL 5PBr Packed Column (3.0 mmI.D. x 100 mm) | End-Fitting: Waters type |
13249-41 | 1 pkg | 541.00 | Buy |
| COSMOSIL 5PBr Packed Column (3.0 mmI.D. x 150 mm) | End-Fitting: Waters type |
13250-01 | 1 pkg | 535.00 | Buy |
| COSMOSIL 5PBr Packed Column (3.0 mmI.D. x 250 mm) | End-Fitting: Waters type |
13251-91 | 1 pkg | 578.00 | Buy |
| COSMOSIL 5PBr Packed Column (4.6 mmI.D. x 50 mm) | End-Fitting: Waters type |
13252-81 | 1 pkg | 573.00 | Buy |
| COSMOSIL 5PBr Packed Column (4.6 mmI.D. x 100 mm) | End-Fitting: Waters type |
12594-41 | 1 pkg | 541.00 | Buy |
| COSMOSIL 5PBr Packed Column (4.6 mmI.D. x 150 mm) | End-Fitting: Waters type |
12394-61 | 1 pkg | 509.00 | Buy |
| COSMOSIL 5PBr Packed Column (4.6 mmI.D. x 250 mm) | End-Fitting: Waters type |
12395-51 | 1 pkg | 552.00 | Buy |
| COSMOSIL 5PBr Packed Column (10.0 mmI.D. x 50 mm) | End-Fitting: Waters type |
13253-71 | 1 pkg | 867.00 | Buy |
| COSMOSIL 5PBr Packed Column (10.0 mmI.D. x 100 mm) | End-Fitting: Waters type |
13254-61 | 1 pkg | 1,006.00 | Buy |
| COSMOSIL 5PBr Packed Column (10.0 mmI.D. x 150 mm) | End-Fitting: Waters type |
13255-51 | 1 pkg | 1,070.00 | Buy |
| COSMOSIL 5PBr Packed Column (10.0 mmI.D. x 250 mm) | End-Fitting: Waters type |
12397-31 | 1 pkg | 1,686.00 | Buy |
| COSMOSIL 5PBr Packed Column (20.0 mmI.D. x 50 mm) | End-Fitting: Waters type |
13257-31 | 1 pkg | 1,755.00 | Buy |
| COSMOSIL 5PBr Packed Column (20.0 mmI.D. x 100 mm) | End-Fitting: Waters type |
13258-21 | 1 pkg | 2,089.00 | Buy |
| COSMOSIL 5PBr Packed Column (20.0 mmI.D. x 150 mm) | End-Fitting: Waters type |
13259-11 | 1 pkg | 2,198.00 | Buy |
| COSMOSIL 5PBr Packed Column (20.0 mmI.D. x 250 mm) | End-Fitting: Waters type |
12398-21 | 1 pkg | 3,933.00 | Buy |
| COSMOSIL 5PBr Packed Column (28.0 mmI.D. x 100 mm) | End-Fitting: Waters type |
13260-71 | 1 pkg | 5,725.00 | Buy |
| COSMOSIL 5PBr Packed Column (28.0 mmI.D. x 150 mm) | End-Fitting: Waters type |
13261-61 | 1 pkg | 6,025.00 | Buy |
| COSMOSIL 5PBr Packed Column (28.0 mmI.D. x 250 mm) | End-Fitting: Waters type |
13262-51 | 1 pkg | 8,079.00 | Buy |
Guard Column / Guard Cartridge
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL 3PBr Guard Cartridge (2.0 mmI.D. x 10 mm ) | End-Fitting: Waters type |
19359-34 | 2 pkg | 236.00 | Buy |
| COSMOSIL 3PBr Guard Cartridge (4.6 mmI.D. x 10 mm ) | End-Fitting: Waters type |
19360-94 | 2 pkg | 236.00 | Buy |
| COSMOSIL 5PBr Guard Cartridge (2.0 mmI.D. x 10 mm) | End-Fitting: Waters type |
18196-94 | 2 pkg | 236.00 | Buy |
| COSMOSIL 5PBr Guard Cartridge (4.6 mmI.D. x 10 mm) | End-Fitting: Waters type |
12444-14 | 2 pkg | 247.00 | Buy |
| COSMOSIL 5PBr Guard Column (10.0 mmI.D. x 20 mm) | End-Fitting: Waters type |
12396-41 | 1 pkg | 594.00 | Buy |
| COSMOSIL 5PBr Guard Column (20.0 mmI.D. x 20 mm) | End-Fitting: Waters type |
13256-41 | 1 pkg | 1,284.00 | Buy |
| COSMOSIL 5PBr Guard Column (28.0 mmI.D. x 50 mm) | End-Fitting: Waters type |
12653-61 | 1 pkg | 3,697.00 | Buy |
HPLC Accessories
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL Direct Cartridge Holder | End-Fitting: Waters type |
19989-71 | 1 pkg | 270.00 | Buy |
| COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) **Discontinued** | End-Fitting: Waters type |
38009-79 | 1 pkg | - | Inquire |
| COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) | End-Fitting: Waters type |
11884-71 | 1 pkg | 265.00 | Buy |
| COSMOSIL Column Prefilter | End-Fitting: Waters type |
39361-19 | 1 pkg | 145.00 | Buy |
| COSMOSIL Column Spare Filter for Prefilter (1 micron) | End-Fitting: Waters type |
39539-09 | 2 pkg | 55.00 | Buy |
| COSMOSIL Column Connecting Tube (0.25 mm) | End-Fitting: Waters type |
37843-69 | 1 pkg | 65.00 | Buy |
| COSMOSIL Column Connecting Tube (0.1 mm) | End-Fitting: Waters type |
12570-41 | 1 pkg | 65.00 | Buy |
#38009-79 has been discontinued. Alternative product is COSMOSIL Direct Cartridge Holder (#19989-71).