COSMOSIL PFP
Pentafluorophenyl-bonded stationary phase
- Pentafluorophenyl-bonded stationary phase
- Alternative selectivity to C18 columns
- Available in analytical and preparative columns
- Suitable for structural isomers and halogenated compounds
| COSMOSIL 5PFP | |
|---|---|
| USP code | L43 |
| Silica gel | High purity porous spherical silica |
| Average particle size | 5 µm |
| Average pore size | approx. 120Å |
| Specific surface area | approx. 300 m²/g |
| Stationary phase | Pentafluorophenyl group |
| Bonding type | Monomeric |
| Endcapping treatment | Yes |
| Carbon content | approx. 10% |
| Usable pH range | 2 - 7.5 |
Alternative Selectivity to C18 Columns
COSMOSIL PFP provides different selectivity from C18 Columns. Furthermore it offers improved separation compared to other companies' PFP columns.
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Separation Properties of PFP
COSMOSIL PFP uses dipole-dipole and π-πinteractions. It offers strong retention for compounds with strong dipole moments, e.g. cationic or halogenated compounds. It also has high steric selectivity for structural isomer separations.
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| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | Methanol : Water = 50 : 50 |
| Flow rate: | 1.0 ml/min |
| Temperature: | 30°C |
| Detection: | UV 254 nm |
| Sample: | o-Dinitrobenzene m-Dinitrobenzene p-Dinitrobenzene Benzene (I.S.) |
Reasonably Priced Preparative Columns
We can offer preparative columns at a reasonable price because we synthesize our own silylating agents.
| Column size | 4.6 mmI.D. x 250 mm | 10 mmI.D. x 250 mm | 20 mmI.D. x 250 mm |
|---|---|---|---|
| HPLC system | Analytical HPLC system | Analytical HPLC system | Semi-preparative HPLC system |
| Flow rate | 1.0 ml/min | 5.0 ml/min | 19.0 ml/min |
| Injection vol. | 10µl | 50µl | 190µl |
| Chromatogram |  |
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| Mobile phase: | Methanol : Water = 70 : 30 |
| Temperature: | 30°C |
| Detection: | UV 254 nm |
| Sample: | 1. Uracil 2. Methyl Benzoate 3. Toluene 4. Naphthalene |
Applications
Fluorinated compounds
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| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | Methanol : Water = 60 : 40 |
| Flow rate: | 1.0 ml/min |
| Temperature: | 30°C |
| Detection: | UV 254 nm |
| Sample: | 1. Benzene (2.5 mg/ml) 2. Fluorobenzene (1.0 mg/ml) 3. Toluene (2.5 mg/ml) 4. α, α, α-Trifluorotoluen (Benzotrifluoride) (0.25 mg/ml) |
| Injection vol.: | 1.0 µl |
Vitamin E
| Column size: | 4.6 mmI.D. x 250 mm |
| Mobile phase: | Methanol : Water = 90 : 10 |
| Flow rate: | 1.0 ml/min |
| Temperature: | 30°C |
| Detection: | UV 295 nm |
| Sample: | 1. δ-Tocotrienol 2. β-Tocotrienol 3. γ-Tocotrienol 4. α-Tocotrienol 5. δ-Tocopherol 6. β-Tocopherol 7. γ-Tocopherol 8. α-Tocopherol |
Comparison of ion-pairing reagents
PFP × Fluorine ion-pairing reagent
Usage Information
| Column | 5 µm PFP | |||||
|---|---|---|---|---|---|---|
| I.D. (mm) | 2.0 | 3.0 | 4.6 | 10.0 | 20.0 | 28.0 |
| Washing method | 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. 2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF. |
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| Storage conditions | Short-term (up to a week): Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. Long-term: Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water. In either case, tightly plug the column, and store in a cool and dry place. |
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| Usable pH range | pH2～pH7.5 | |||||
| Max. pressure | 20MPa | 15MPa | ||||
| Temperature range | The maximum usable temperature is 60℃. However. for regular use, please use at a constant temperature between 20°C and 50°C. | |||||
| Usable solvents | Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable. Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points. |
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| Other instructions | - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L. - Always filter mobile phases using a 0.5 µm or finer filter before use. - Acetonitrile is not recommended as a mobile phase. |
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Downloads
Brochures
- COSMOSIL 5PFP
(1.5 MB)
COSMOSIL Application Search
COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size
Ordering Information
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL PFP Packed Column (2.0 mm I.D. x 50 mm) | End-Fitting: Waters type |
13263-41 | 1 pkg | 428.00 | Buy |
| COSMOSIL PFP Packed Column (2.0 mm I.D. x 100 mm) | End-Fitting: Waters type |
13264-31 | 1 pkg | 535.00 | Buy |
| COSMOSIL PFP Packed Column (2.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
12381-21 | 1 pkg | 621.00 | Buy |
| COSMOSIL PFP Packed Column (2.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
13265-21 | 1 pkg | 717.00 | Buy |
| COSMOSIL PFP Packed Column (3.0 mm I.D. x 50 mm) | End-Fitting: Waters type |
13266-11 | 1 pkg | 461.00 | Buy |
| COSMOSIL PFP Packed Column (3.0 mm I.D. x 100 mm) | End-Fitting: Waters type |
13267-01 | 1 pkg | 535.00 | Buy |
| COSMOSIL PFP Packed Column (3.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
13268-91 | 1 pkg | 514.00 | Buy |
| COSMOSIL PFP Packed Column (3.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
13269-81 | 1 pkg | 675.00 | Buy |
| COSMOSIL PFP Packed Column (4.6 mm I.D. x 50 mm) | End-Fitting: Waters type |
13270-41 | 1 pkg | 461.00 | Buy |
| COSMOSIL PFP Packed Column (4.6 mm I.D. x 100 mm) | End-Fitting: Waters type |
13271-31 | 1 pkg | 535.00 | Buy |
| COSMOSIL PFP Packed Column (4.6 mm I.D. x 150 mm) | End-Fitting: Waters type |
12383-01 | 1 pkg | 541.00 | Buy |
| COSMOSIL PFP Packed Column (4.6 mm I.D. x 250 mm) | End-Fitting: Waters type |
12384-91 | 1 pkg | 675.00 | Buy |
| COSMOSIL PFP Packed Column (10.0 mm I.D. x 50 mm) | End-Fitting: Waters type |
13272-21 | 1 pkg | 856.00 | Buy |
| COSMOSIL PFP Packed Column (10.0 mm I.D. x 100 mm) | End-Fitting: Waters type |
13273-11 | 1 pkg | 1,103.00 | Buy |
| COSMOSIL PFP Packed Column (10.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
13274-01 | 1 pkg | 1,199.00 | Buy |
| COSMOSIL PFP Packed Column (10.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
12386-71 | 1 pkg | 1,541.00 | Buy |
| COSMOSIL PFP Packed Column (20.0 mm I.D. x 50 mm) | End-Fitting: Waters type |
13276-81 | 1 pkg | 1,588.00 | Buy |
| COSMOSIL PFP Packed Column (20.0 mm I.D. x 100 mm) | End-Fitting: Waters type |
13277-71 | 1 pkg | 2,568.00 | Buy |
| COSMOSIL PFP Packed Column (20.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
13278-61 | 1 pkg | 2,697.00 | Buy |
| COSMOSIL PFP Packed Column (20.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
12387-61 | 1 pkg | 3,585.00 | Buy |
| COSMOSIL PFP Packed Column (28.0 mm I.D. x 100 mm) | End-Fitting: Waters type |
13280-11 | 1 pkg | 5,457.00 | Buy |
| COSMOSIL PFP Packed Column (28.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
13281-01 | 1 pkg | 5,778.00 | Buy |
| COSMOSIL PFP Packed Column (28.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
13282-91 | 1 pkg | 7,704.00 | Buy |
Guard Column / Guard Cartridge
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL PFP Guard Cartridge (4.6 mm I.D. x 10 mm) | End-Fitting: Waters type |
12443-24 | 2 pkg | 225.00 | Buy |
| COSMOSIL PFP Guard Column (10.0 mm I.D. x 20 mm) | End-Fitting: Waters type |
12385-81 | 1 pkg | 541.00 | Buy |
| COSMOSIL PFP Guard Column (20.0 mm I.D. x 20 mm) | End-Fitting: Waters type |
13275-91 | 1 pkg | 1,284.00 | Buy |
| COSMOSIL PFP Guard Column (28.0 mm I.D. x 20 mm) | End-Fitting: Waters type |
13279-51 | 1 pkg | 4,494.00 | Buy |
HPLC Accessories
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL Direct Cartridge Holder | End-Fitting: Waters type |
19989-71 | 1 pkg | 270.00 | Buy |
| COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) **Discontinued** | End-Fitting: Waters type |
38009-79 | 1 pkg | - | Inquire |
| COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) | End-Fitting: Waters type |
11884-71 | 1 pkg | 265.00 | Buy |
| COSMOSIL Column Prefilter | End-Fitting: Waters type |
39361-19 | 1 pkg | 145.00 | Buy |
| COSMOSIL Column Spare Filter for Prefilter (1 micron) | End-Fitting: Waters type |
39539-09 | 2 pkg | 55.00 | Buy |
| COSMOSIL Column Connecting Tube (0.25 mm) | End-Fitting: Waters type |
37843-69 | 1 pkg | 65.00 | Buy |
| COSMOSIL Column Connecting Tube (0.1 mm) | End-Fitting: Waters type |
12570-41 | 1 pkg | 65.00 | Buy |
#38009-79 has been discontinued. Alternative product is COSMOSIL Direct Cartridge Holder (#19989-71).