COSMOCORE 2.6Cholester
Core-Shell Particle
Features
- Cholesterol-bonded reversed phase core-shell column
- Use under the same condition as C18 columns
- Better selectivity for cis-trans isomers, polyphenols, and natural products
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Comparison with C18
COSMOCORE 2.6Cholester offers improved separation for cis-trans isomers than C18 under typical reversed-phase mobile phase.
| Column size: | 2.1 mmI.D. x 100 mm |
| Mobile phase: | Methanol |
| Flow rate: | 0.4 ml/min |
| Temperature: | 40°C |
| Detection: | UV 280 nm |
| Sample: | 1. Vitamin K2 (0.50 mg/ml) 2. Trans-Vitamin K1 (0.50 mg/ml) 3. Cis-Vitamin K1 |
| Inj. Vol.: | 0.5 µl |
Separation Properties
COSMOCRE 2.6Cholester has the same hydrophobicity as C18. It is not necessary to change the analytical conditions when replacing C18 Columns with COSMOCORE 2.6Cholester.
| Column size: | 2.1 mmI.D. x 100 mm |
| Mobile phase: | Acetonitrile : H2O = 50 : 50 |
| Flow rate: | 0.4 ml/min |
| Temperature: | 40°C |
| Detection: | UV 254 nm |
| Sample: | 1. Uracil 2. Methyl Benzoate 3. Toluene 4. Naphthalene |
| Inj. Vol.: | 0.25 µl |
Molecular Shape Selectivity
COSMOCORE 2.6Cholester has excellent shape slectivity due to its structural rigidity. COSMOCORE 2.6Cholester retains planar Triphenylene longer than Triphenylene stereoscopic o-Terphenyl.
| Column size: | 2.1 mmI.D. x 100 mm |
| Mobile phase: | Acetonitrile : H2O = 70 : 30 |
| Flow rate: | 0.4 ml/min |
| Temperature: | 40°C |
| Detection: | UV 254 nm |
| Sample: | 1. o-Terphenyl (0.25 mg/ml) 2. Triphenylene (0.50 mg/ml) |
| Inj. Vol.: | 0.5 µl |
Scaling Up from Analytical to Preparative Separation
COSMOCORE 2.6Cholestrer has the same functional group as the fully-porous COSMOSIL Cholester 5 µm silica packing material. Therefore, the separation pattern in COSMOCORE 2.6Cholester is maintained when scaling up to preparative separation with 5 µm COSMOSIL Cholester.
| Mobile phase: | Methanol |
| Flow rate: | (2.1 mmI.D.) 0.4 ml/min (4.6 mmI.D.) 1.0 ml/min (10.0 mmI.D.) 5.0 ml/min |
| Temperature: | 40°C |
| Detection: | UV 280 nm |
| Sample: | 1. trans-Vitamin K1 2. cis-Vitamin K1 |
Application: Separation of natural components (Tomato Components)
Lycopene exists in many cis-trans isomers. COSMOCORE 2.6Cholester separates these compounds better than C18.
| Column size: | 3.0 mmI.D. x 50 mm |
| Mobile phase: | (2.6C18) Acetonitrile : H2O = 95 : 5 (2.6Cholester) Acetonitrile |
| Flow rate: | 1.0 ml/min |
| Temperature: | 40°C |
| Detection: | UV 470 nm |
| Sample: | 1. Lycopene 2. β-Carotene |
| Inj. Vol.: | 0.5 µl |
Application: Separation of Catechin
| Column size: | 2.1 mmI.D. x 50 mm |
| Mobile phase: | A; Acetonitrile : 0.1% H3PO4 = 10 : 90 B; Acetonitrile : 0.1% H3PO4 = 40 : 60 B conc. 0 → 100% 3 min Linear gradient |
| Flow rate: | 0.6 ml/min |
| Temperature: | 30°C |
| Detection: | UV 280 nm |
| Sample: | 1. (-)-Gallocatechin[(-)-GC] 2. Caffeine 3. (-)-Epigallocatechin[(-)-EGC] 4. (-)-Catechin[(-)-C] 5. (-)-Epicatechin[(-)-EC] 6. (-)-Epigallocatechin G allate[(-)-EGCg] 7. (-)-Gallocatechin Gallate[(-)-GCg] 8. (-)-Epicatechin Gallate[(-)-ECg] 9. (-)-Catechin Gallat[(-)-Cg] |
Poster: Differentiate Δ9-THC from Δ8-THC by Reversed-Phase Core-Shell HPLC Column
Click here to download (pdf)
Applications
Oligonucleotides
Catechins
The number of reference for COSMOSIL Cholesterol Bonded Stationary Phase
The number of COSMOSIL Cholester reference has grown to over 100 since its introduction in 2005.
(Reference Search: Google Scholar, as of July 2014)
Figure 1: The number of reference of COSMOSIL Cholester and 2.5Cholester
» Information on COSMOSIL Cholester (5 µm)
» Information on COSMOSIL 2.5Cholester (2.5 µm)
Usage information
| Column | 2.6 COSMOCORE Cholester | ||
|---|---|---|---|
| I.D. (mm) | 2.1 | 3 | 4.6 |
| Washing method | 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. 2. (If you used a buffer, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with methanol and/or THF. |
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| Storage conditions | Short-term (up to a week): Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. Long-term: Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water. In either case, tightly plug the column, and store in a cool and dry place. |
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| Usable pH range | pH 2-7.5 | ||
| Max. pressure | 60MPa | ||
| Temperature range | The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C. | ||
| Usable solvents | Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable. Note: For solvents with high viscosity, please watch the system pressure and keep it below 60 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points. |
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| Other instructions | - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L. - Always filter mobile phases using a 0.45 µm or finer filter before use. |
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Ordering Information
Column
| Product | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|
| COSMOCORE 2.6Cholester Packed Column, 2.1mmI.D. x 30mm | 12858-91 | 1 pkg | 697.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 2.1mmI.D. x 50mm | 12859-81 | 1 pkg | 712.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 2.1mmI.D. x 75mm | 12860-41 | 1 pkg | 798.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 2.1mmI.D. x 100mm | 12861-31 | 1 pkg | 814.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 2.1mmI.D. x 150mm | 12862-21 | 1 pkg | 867.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 3.0mmI.D. x 30mm | 12863-11 | 1 pkg | 697.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 3.0mmI.D. x 50mm | 12864-01 | 1 pkg | 712.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 3.0mmI.D. x 75mm | 12866-81 | 1 pkg | 798.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 3.0mmI.D. x 100mm | 12867-71 | 1 pkg | 814.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 3.0mmI.D. x 150mm | 12868-61 | 1 pkg | 867.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 30mm | 12869-51 | 1 pkg | 711.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 50mm | 12870-11 | 1 pkg | 712.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 75mm | 12871-01 | 1 pkg | 798.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 100mm | 12872-91 | 1 pkg | 814.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 150mm | 12873-81 | 1 pkg | 873.00 | Buy |
| COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 250mm | 12875-61 | 1 pkg | 1,110.00 | Buy |
Prefilter
| Product | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|
| U-Fil UHPLC-compatible prefilter | 12571-31 | 1 pkg | 160.00 | Buy |