Recombinant Human FGF-basic, Animal-free
| Recombinant human FGF-basic (bFGF) (AA 1-154), also called as FGF-2 or FGFb, is a bioactive protein intended for use in cell culture applications. bFGF is a heparin-binding member of the FGF superfamily of molecules. It is involved in a number of biological processes including embryonic development, differentiation, survival, regeneration and migration. In addition, bFGF is a critical factor for growing embryonic stem cells in culture to remain cell in an undifferentiated state. |  |
| Source: | Escherichia Coli. |
| Purity: |
Greater than 98%, as determined by SDS-PAGE (See SDS-PAGE Image)
 |
| Endotoxin: | Less than 0.01 ng/ug cytokine as determined by the LAL assay |
| Bioactivity: | ED50= 0.1-0.3 ng/ml as determined by the dose dependent proliferation of NIH 3T3 cells. |
| Formulation: | Sterile filtered and lyophilized from Tris buffer (10 mM Tris pH7.5, 150 mM NaCl). |
| Reconstitution: | Centrifuge vial before opening. It is recommended to reconstitute bFGF in sterile 10 mM Tris-HCl, pH7.5 to yield a stock solution of 0.1 mg/ml of bFGF. It is stable for up to 6 months when stored at -20°C and up to 12 months when stored at -80°C. Multiple freeze/thaw cycles will result in significant loss of activity. |
Cell Culture of NIH/3T3 Cells
 NIH/3T3 cells (mouse fibroblast cell line, ATCC: CCL-1668) were cultured in the absence of bFGF. |
 NIH/3T3 cells were cultured with 0.25 ng/ml bFGF in serum-free medium at 37°C, 5% CO2 for 24 hours. |
Bioactivity assay
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Recombinant Proteins (brochure)Ordering Information
| Product | Storage | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| Recombinant Human FGF-basic, Animal-free (Lyophilized) | -20°C | NU0005-1 | 10 µg | 99.00 | Buy |
| Recombinant Human FGF-basic, Animal-free (Lyophilized) | -20°C | NU0005-3 | 50 µg | 215.00 | Buy |
| Recombinant Human FGF-basic, Animal-free (Lyophilized) | -20°C | NU0005-6 | 1 mg | 1,213.00 | Buy |
| Recombinant Human FGF-basic, Animal-free (Liquid Form) | -20°C | NU0005-6L | 1 mg | 1,213.00 | Buy |