Recombinant Human CDK2/Cyclin E1 Complex
Cyclin-dependent kinase 2, p33
| CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1/S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition. Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
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Synonym(s):
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Cyclin-dependent kinase 2, 933
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Molecular Weight :
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91 kDa (complex), 60.2 kDa (CDK2), 73.4 kDa (Cyclin E1)
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Tag :
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N-GST (CDK2), N-6xHis (Cyclin E1)
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Source :
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Sf9 insect cells
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Purity :
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Greater than 95%, as determined by SDS-PAGE
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| Activity : |  |
| Concentration : | 0.5 mg/ml |
| Formulation : |
50 mM TrisCl pH 7.5, 150 mM NaCl, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 10 mM Glutathione, 25% Glycerol
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| GeneBank Accession # : |
NM_001798.5, a.a. 1-298 (CDK2)
NM_001238.4, a.a. 1-410 (Cyclin E1)
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| Application : | Inhibitor Screening, Kinding Assays |
| Storage : | Stable for 12 months at -80°C, Avoid freeze/thaw cycles |
| Reference : |
Levkau, B. et al: Cleavage of p21(Cip1/Waf1) and p27(Kip1) mediates apoptosis in endothelial cells through activation of Cdk2: role of a caspase cascade. Molec. Cell1: 553‐563, 1998.
Huang, H. et al: CDK2‐dependent phosphorylation of FOXO1 as an apoptotic response to DNA damage. Science314: 294‐297, 2006.
Ohtsubo, M., et al., Mol Cell Biol. 1995;15(5):2612-2624.
Harbour, J.W., et al., Cell. 1999;98(6):859-869.
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Recombinant Proteins (Brochure)