Recombinant Human CDK2/Cyclin A2 Complex
Cyclin-dependent Kinase 2, cyclin A
| A member of the CMGC class of serine/threonine protein kinases, acting in cell cycle progression. Cyclin A2 stimulates CDK2 kinase activity upon complex formation |
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Synonym(s):
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Cyclin-dependent kinase 2, cyclin A
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Molecular Weight :
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60.7 kDa (CDK2). 52.5 kDa (Cyclin A2)
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Tag :
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N-GST (CDK2), N-6xHis (Cyclin A2)
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Source :
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Sf9 insect cells
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Purity :
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Greater than 90%, as determined by SDS-PAGE
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Activity:
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Concentration :
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0.5 mg/ml
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Formulation :
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50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 10 mM Glutathione, 25% Glycerol
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GeneBank Accession # :
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NM_001798.5, a.a. 1-298 (CDK2)
NM_001237.5, a.a. 1-432 (Cyclin A2)
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Application :
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Inhibitor Screening, Kinase Assays
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Storage :
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Stable for 12 months at -80°C, Avoid freeze/thaw cycles
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Reference :
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Pagano, M., et al., EMBO J. 1992;11(3):961-71.
Brown NR., et al., Cell Cycle. 2007 Jun 1,6(11):1350-9.
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Recombinant Proteins (Brochure)