Protein extraction by using Cell Lysis Buffer or other company’s RIPA Buffer

CBB Staining

Protein was extracted from rat kidney by using this product or Company A’s RIPA Buffer, then subjected to electrophoresis, and CBB staining. The result shows protein extraction efficiency of this product is equal to that of Company A’s RIPA Buffer.

Conditions：

 Western Blotting

Protein was extracted from mouse liver by using this product or Company A’s RIPA Buffer, then subjected to western blotting, and CBB stained. The results show that this product is applicable to western blotting, similar to Company A’s RIPA Buffer.

Conditions:

Phosphorylated protein detection by using extracts from Cell Lysis Buffer

Protein was extracted from Protein Kinase C (PKC)-activated HEK293 cells by using this product, and ERK and pERK were detected by Western blotting.

Conditions:

Protein extraction by using Cell Lysis Buffer (10x)

CBB Staining

Protein was extracted from various mouse organs by using this product with SDS, subject to electrophoresis and CBB staining.

Conditions:

Immunoprecipitation

Protein was extracted from GFP-expressing HEK293 cells using this product without SDS and immunoprecipitated using rat anti-GFP antibody-conjugated agarose beads. GFP was detected by Western blotting of the samples before and after immunoprecipitation. GFP in the samples before and after immunoprecipitation was detected by Western blotting.

Conditions:

 Cell Lysis Buffer (10X) (Instruction)

 Cell Lysis Buffer (Instruction)

 RIPA Buffer (10X) (Instruction)

1. Yehya, Ashwaq HS, et al. "Polymolecular botanical drug of Orthosiphon stamineus extract (C5OSEW5050ESA) as a complementary therapy to overcome gemcitabine resistance in pancreatic cancer cells." Journal of Traditional and Complementary Medicine 13.1 (2023): 39-50.

2. Kameda, Shigetoshi, Hirohisa Ohno, and Hirohide Saito. "Synthetic circular RNA switches and circuits that control protein expression in mammalian cells." Nucleic Acids Research (2023).

3. Hironaka, Takanori, et al. "The well-developed actin cytoskeleton and Cthrc1 expression by actin-binding protein drebrin in myofibroblasts promote cardiac and hepatic fibrosis." Journal of Biological Chemistry (2023): 102934.