The product inhibits Ser/Thr phosphatase, Tyrosine Phosphatase, Acid Phosphatase and Alkaline Phosphatase. The product preserves small quantity of phosphorylated protein.

Phosphatase Inhibitor Cocktail (EDTA free) (100X) (Cat. No.: 07575-51)

 

The product (100 times concentrated stock solution) contains:

Inhibitors	Targeted phosphatase
Imidazole	Alkaline Phosphatase
Sodium Orthovanadate (V)	Protein Tyrosine Phosphatase Alkaline Phosphatase
b-Glycerophosphoric Acid Disodium Salt	Ser/Ther Phosphatase
Sodium (+)-Tartrate Dihydrate	Acid Phosphatase
Sodium Fluoride	Acid Phosphatase Protein Phosphatase-2A Protein Phosphatase-2B Protein Phosphatase-2C
Disodium Molybdate (VI) Dihydrate	Acid Phosphatase Phosphoprotein phosphatase

 

Phosphatase Inhibitor Cocktail (Cat. No.: 07574-61)

The product (100 times concentrated stock solution) contains:

Inhibitors	Targeted phosphatase
Imidazole	Alkaline Phosphatase
tetra-Sodium Ethylenediaminetetraacetate	Alkaline Phosphatase Protein Phosphatase-2B Protein Phosphatase-2C
Sodium Orthovanadate (V)	Protein Tyrosine Phosphatase Alkaline Phosphatase
β-Glycerophosphoric Acid Disodium Salt	Ser/Thr Phosphatase
Sodium (+)-Tartrate Dihydrate	Acid Phosphatase
Sodium Fluoride	Acid Phosphatase Protein Phosphatase-2A Protein Phosphatase-2B Protein Phosphatase-2C
Disodium Molybdate(VI) Dihydrate	Acid Phosphatase Phosphoprotein phosphatase

Dilute 100 times with any buffer of your choice.

 Phosphatase Inhibitor Cocktail (Instruction)

 Phosphatase Inhibitor Cocktail (EDTA free) (100X) (Instruction)

 Phosphatase Inhibitor Cocktail (Brochure)

• Kosakamoto, Hina, et al. "Sensing of the non-essential amino acid tyrosine governs the response to protein restriction in Drosophila." Nature Metabolism 4.7 (2022): 944-959.

• Hara, Yuta, et al. "Anti‐tumor effects of an antagonistic mAb against the ASCT2 amino acid transporter on KRAS‐mutated human colorectal cancer cells." Cancer Medicine 9.1 (2020): 302-312.

• Ueda, Yohei, et al. "Exogenous C-type natriuretic peptide therapy for impaired skeletal growth in a murine model of glucocorticoid treatment." Scientific Reports 9.1 (2019): 8547.

Phosphatase Inhibitor Cocktail (Cat. No.: 07574-61)

• In case of very high phosphatase concentration, use higher concentration of the product (dilute from 5 to 20 times).
• The product may cause inhibition of metal chelate affinity chromatography and 2D electrophoresis. Remove inhibitors by dialysis or desalting before performing such procedures.
• The product does not contain protease inhibitors. For homogenizing cells and tissues, if necessary, add protease inhibitor cocktail, such as Protease Inhibitor Cocktail (Code No.: 25955-11, Code No.: 03969-21 and Code No.: 04080-11).

Phosphatase Inhibitor Cocktail (EDTA free) (100X) (Cat. No.: 07575-51)

• If phosphatase concentration is high, it can be diluted by 20-50 fold.
• The product may cause inhibition of 2D electrophoresis. Remove inhibitors by dialysis or desalt before use.
• The product does not contain protease inhibitors. For homogenizing cells and tissues, if necessary, add protease inhibitor cocktail, such as Protease Inhibitor Cocktail (EDTA free) (Cat. No.: 03969).
• The product includes Imidazole for inhibitor. In case of Metal Affinity Purification, it is recommended a pretest with reducing Imidazole concentration in the buffer by 5 mM.
• The product colors gradually.
• Direct light may interfere with an inhibition effect; shield the product from the light after using.
• The components may be precipitated during storage. Before use, melt it by vortex at 30-40°C shielding light.