COSMOSIL 3C18-SS
Perfecting Chemical Modification Technology
COSMOSIL 3C18-SS is the product of perfecting our chemical modification and endcapping technology, enabling this column to withstand alkaline and acidic mobile phases. It also resists peak tailing, and with the 3 μm particle size, fast and efficient analysis is possible on conventional instruments. It is recommended as a general-use column, especially for method development in which the analysis conditions are uncertain.
Applications
Oligonucleotides (basic mobile phase, high temperature)
By using an alkaline mobile phase under high temperatures with LC-MS, impurities from the oligonucleotide synthesis process (short-mers and P-S to P-O conversion impurities) could be separated from the full-length product.
Peptides (acidic mobile phase, high temperature)
Using standard peptide analysis conditions, these 3 structurally similar compounds (α-defensin) could not be separated. However, by changing the mobile phase additive to formic acid and increasing the temperature, resolution (RS) of greater than 1.5 was achieved.
Comparison of durability with basic mobile phase
When used with a harshly basic mobile phase, most C18 columns degrade quickly. The below graphs show the retention time (tr) and theoretical plates (N), respectively, of the COSMOSIL 3C18-SS and two other columns under our accelerated test.
Under the highly basic mobile phase conditions above, COSMOSIL 3C18-SS showed high durability. This column can be used under harsh conditions, overcoming the limitations of conventional silica-based columns.
Comparison of separation performance
COSMOSIL 3C18-SS was compared to hybrid silica and core-shell columns. Overall, COSMOSIL 3C18-SS demonstrated high efficiency and low backpressure, in addition to sharp peaks even for basic and metal-coordination compounds.
Retention behavior
Bsic compound
Metal coordination compound
Basic properties
| COSMOSIL 3C18-SS | |
|---|---|
| USP code | L1 |
| Silica gel | High purity fully-porous spherical silica gel |
| Average particle size | Approx. 3 μm |
| Average pore size | Approx. 12 μm |
| Specific surface area | Approx. 300m2/g |
| Stationary phase | Octadecyl group |
| Maximum pressure | 30 MPa |
| pH range | 1 - 12＊ |
＊ For longest lifetime, pH 2-10 is recommended.
Ordering Information
3 μm particle size
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL 3C18-SS Packed Column (2.0 mmI.D. x 50 mm) | End-Fitting: Waters type | 22560-61 | 1 PKG | 560.00 | Buy |
| COSMOSIL 3C18-SS Packed Column (2.0 mmI.D. x 75 mm) | End-Fitting: Waters type | 22542-01 | 1 PKG | 560.00 | Buy |
| COSMOSIL 3C18-SS Packed Column (2.0 mmI.D. x 100 mm) | End-Fitting: Waters type | 22543-91 | 1 PKG | 560.00 | Buy |
| COSMOSIL 3C18-SS Packed Column (2.0 mmI.D. x 150 mm) | End-Fitting: Waters type | 22546-61 | 1 PKG | 620.00 | Buy |
| COSMOSIL 3C18-SS Packed Column (2.0 mmI.D. x 250 mm) | End-Fitting: Waters type | 22547-51 | 1 PKG | 750.00 | Buy |
| COSMOSIL 3C18-SS Packed Column (3.0 mmI.D. x 50 mm) | End-Fitting: Waters type | 22548-41 | 1 PKG | 560.00 | Buy |
| COSMOSIL 3C18-SS Packed Column (3.0 mmI.D. x 75 mm) | End-Fitting: Waters type | 22549-31 | 1 PKG | 560.00 | Buy |
| COSMOSIL 3C18-SS Packed Column (3.0 mmI.D. x 100 mm) | End-Fitting: Waters type | 22550-91 | 1 PKG | 560.00 | Buy |
| COSMOSIL 3C18-SS Packed Column (3.0 mmI.D. x 150 mm) | End-Fitting: Waters type | 22551-81 | 1 PKG | 620.00 | Buy |
| COSMOSIL 3C18-SS Packed Column (3.0 mmI.D. x 250 mm) | End-Fitting: Waters type | 22552-71 | 1 PKG | 750.00 | Buy |
| COSMOSIL 3C18-SS Packed Column (4.6 mmI.D. x 50 mm) | End-Fitting: Waters type | 22553-61 | 1 PKG | 560.00 | Buy |
| COSMOSIL 3C18-SS Packed Column (4.6 mmI.D. x 75 mm) | End-Fitting: Waters type | 22554-51 | 1 PKG | 560.00 | Buy |
| COSMOSIL 3C18-SS Packed Column (4.6 mmI.D. x 100 mm) | End-Fitting: Waters type | 22555-41 | 1 PKG | 560.00 | Buy |
| COSMOSIL 3C18-SS Packed Column (4.6 mmI.D. x 150 mm) | End-Fitting: Waters type | 22558-11 | 1 PKG | 620.00 | Buy |
| COSMOSIL 3C18-SS Packed Column (4.6 mmI.D. x 250 mm) | End-Fitting: Waters type | 22559-01 | 1 PKG | 750.00 | Buy |
Guard Cartridge
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL 3C18-SS Guard Cartridge (2.0 mmI.D. x 10 mm) | End-Fitting: Waters type | 22539-74 | 2 PKG | 255.00 | Buy |
| COSMOSIL 3C18-SS Guard Cartridge (4.6 mmI.D. x 10 mm) | End-Fitting: Waters type | 22540-34 | 2 PKG | 255.00 | Buy |
HPLC Accessories
| Product | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|
| COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) | 11884-71 | 1 pkg | 265.00 | Buy |
| COSMOSIL Direct Cartridge Holder (4.6 mm I.D.) | 19989-71 | 1 pkg | 270.00 | Buy |
| COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) **Discontinued** | 38009-79 | 1 pkg | - | Inquire |
| COSMOSIL Column Spare Filter for Prefilter (1 micron) | 39539-09 | 2 pkg | 55.00 | Buy |
| COSMOSIL Column Connecting Tube (0.25 mm) | 37843-69 | 1 pkg | 65.00 | Buy |
| COSMOSIL Column Connecting Tube (0.1 mm) | 12570-41 | 1 pkg | 65.00 | Buy |
| COSMOSIL Column Prefilter | 39361-19 | 1 pkg | 145.00 | Buy |
** Guard cartridges require a guard cartridge holder.
** #38009-79 has been discontinued. Alternative product is COSMOSIL Direct Cartridge Holder (#19989-71).