BioMasher II consists of a 1.5 mL microtube and a pestle. The sample and extraction buffer are placed in the tube, and ground to prepare the homogenate. The microtube and pestle are molded so that they fit without any space between them. The texture of the inner wall of the tube and the outer periphery of the tip of the pestle enhances the efficiency of the disruption of the sample. The pestle has a splash guard, which is designed to fit the tube without unnecessary spaces. BioMasher II can be used to disrupt various samples, such as animal tissue/organ, plants (stems, roots, seeds), and insects.

• For samples that are difficult to disrupt, an exclusive electric stirrer (Nippi PowerMasher II) is available for easy disruption.

• DO NOT autoclave BioMasher II. In the case sterilization is required, please use a sterilized product.

BioMasher III has the features of both BioMasher I and II. This model consists of a filter tube with an abrasive surface inner wall and a pestle with a textured surface. After grinding the sample in the filter tube using the pestle, centrifuge the extraction liquid. The extracted sample will be filtered and caught in the tube. Since the fibrous membrane component get trapped by the filter, BioMasher III is recommended for homogenization of highly fibrous samples.

• For samples that are difficult to homogenize, an exclusive electric stirrer (Nippi PowerMasher II) can be used for ease.

BioMasher V is a larger volume version of BioMasher III. After homogenizing the sample with a combination of the tube with a textured inner wall and the stirring pestle with an abrasive surface, centrifuge and filtrate with the filter. Recommended for homogenizing highly fibrous samples.

• For samples difficult to homogenize, an exclusive electric stirrer (PowerMasher II) is available for ease.

BioMasher SP is a larger volume version of BioMasher II. This model has enhanced disruption efficacy from the combination of the 15 mL tube with an abrasive inner wall and a stirring pestle with a textured tip. The shovel-like shape cutout and the helical groove of the pestle effectively trap and homogenize the tissue. BioMasher SP is suitable for disruption of animal tissue/organ, plants (stems, roots, seeds), and insects.

• For samples difficult to disrupt, an exclusive electric stirrer (Nippi PowerMasher II) is available for easy disruption.
• DO NOT autoclave BioMasher II. In the case sterilization is required, please use a sterilized product.

PowerMasher II helps the disruption of large amounts of samples and disruption that is difficult to complete manually. The device is attached to the stirring pestle of the BioMasher II, III, V, and SP to allow electrical stirring.
Geared high torque type with a rotation speed of 450 rpm.

•  Biomasher Users Manual
•  BiomasherTechnicalDocument

Q1. In terms of sample volume, which model of the BioMasher is appropriate?
→ Our recommendation is as below. 
Sample size
BM I: 50 mg
BM II: 100 mg
BM III: 50 mg
BM V: 500 mg
BM SP: 1,000 mg

Q2. What is the centrifugation resistance of the BioMasher?
→ Although it depends on sample, Please try the following conditions as a standard.
BM I: 10,000-15,000 g, 10-30 seconds
BM II: 10,000-15,000 g, 10-30 seconds
BM III: 6,000-10,000 g, 10-30 seconds
BM V: 3,000-6,000 g, 5 minutes
BM SP: 3,000-6,000 g, 5 minutes

Q3. What is the lowest temperature BioMasher can bear?
→ The material of the tube is PP, and its freezing resistance is approximately -60°C to -40°C. Cooling of the sample with liquid nitrogen together with the tube may cause damage.