Recombinant FGF-2 TOP^® is produced by recombinant expression of the human sequence of FGF-2 in plant seeds of Camelina sativa. Modifications to the sequence for improved expression, bioactivity and stability are applied but are proprietary. None of the components or raw materials employed for the production of FGF-2 TOP^® are derived or extract from animal or human origin.

Uniquely Engineered for Stem Cell Production:

FGF-2 TOP^® engineered with a novel nine amino acid substitution to improve stability is thermostabilized growth factor that enables more efficient FGF-2-dependent cell cultures with fewer media changes.

Wild Type FGF-2	FGF-2 TOP®

Product Information:

Specifications: 

FGF-2 TOP® (Thermostability Optimized) has an expected ED50 of less than 0.1 ng/mL, as determined by NIH-3T3 cell proliferation assay. The ED50 value of FGF-2 TOP® is about 5-times lower than that of the wild-type FGF-2, as determined by a NIH/3T3 proliferation assay. Under the same experimental conditions, less FGF-2 TOP® is needed to achieve a given effect on cell proliferation, thanks to the higher stability and prolonged half-life of FGF-2 TOP®. Thanks to this competitive advantage, lower dosage of FGF-2 are required in your cell culture applications (up to 10x).

FGF-2 TOP® is a stabilized growth factor that offers a novel way to grow FGF-2 dependent cell cultures more efficiently, with fewer media changes. FGF-2 TOP® retains full biological activity even after twenty days at 37°C. The stable level of FGF-2 in culture allows for a more homogenous, undifferentiated pluripotent stem cell cultures, and consistent downstream differentiations, while saving researchers valuable time and money because repeated supplementation with FGF-2 and a daily medium change is not required.

Realize tangible benefits of FGF-2 TOP® protein stability: significant reductions in the amount of media required to feed cells, as well as reductions in the number of feedings, saving labor costs and obviating the need for inconvenient weekend feedings. Refer to the example feeding schedule and cost comparison of wild-type versus TOP®:

• FGF2-G3 was developed by Dvorak et al., 2018. Biotechnology and Bioengineering

Dvorak, P., Bednar, D., Vanacek, P., Balek, L., Eiselleova, L., Stepankova, V., Sebestova, E., Kunova Bosakova, M., Konecna, Z., Mazurenko, S. and Kunka, A., 2018. Computer‐assisted engineering of hyperstable fibroblast growth factor 2. Biotechnology and bioengineering, 115(4), pp.850-862.

• B8 media protocol established by Kuo et al., 2020. Stem Cell Reports 

Kuo, H.H., Gao, X., DeKeyser, J.M., Fetterman, K.A., Pinheiro, E.A., Weddle, C.J., Fonoudi, H., Orman, M.V., Romero-Tejeda, M., Jouni, M. and Blancard, M., 2020. Negligible-cost and weekend-free chemically defined human iPSC culture. Stem Cell Reports, 14(2), pp.256-270.

• updated in Lyra-Leite et al., 2021. STAR Protocols 

Lyra-Leite, D.M., Fonoudi, H., Gharib, M. and Burridge, P.W., 2021. An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells. STAR protocols, 2(1), p.100213.

• Review of FGF 2 stabilization by Benington et al., 2020. Pharmaceutics

Benington, L., Rajan, G., Locher, C. and Lim, L.Y., 2020. Fibroblast growth factor 2—A review of stabilisation approaches for clinical applications. Pharmaceutics, 12(6), p.508.

• Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat, Kaplan et al., 2021.

Stout, A.J., Mirliani, A.B., Rittenberg, M.L., Shub, M., White, E.C., Yuen Jr, J.S. and Kaplan, D.L., 2022. Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat. Communications Biology, 5(1), p.466.

 Thermostable Recombinant FGF-2 (FGF-2 STAB) (Product Information)

 Thermostable Recombinant FGF-2 (FGF-2 STAB) (Brochure)

Related Product: 

 FGF-2 Human (Product Information)

 FGF-2 Bovine (Product Information)