Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate, the last reaction of glycolysis. The M2 isozyme of pyruvate kinase, or PKM2 is the only isozyme expressed in cancer cells, and have been used clinically as tumor maker for detection of early cancer and monitor progress of cancer treatment.

Molecular Weight :	60 kDa
Source :	E. coli
Purification:	Sequential chromatography
Purity :	Greater than 90%, as determined by SDS-PAGE
Formulation :	0.5 mg/ml PKM2 with 2 mM DTT, 20% glycerol, 0.02% NaN3, 150 mM NaCl, 0.1 mM EDTA, 50 mM Tris-HCl pH7.5.
Storage :	Upon delivery aliquot and store at -80 °C. Avoid multiple freeze-thaw cycles. The protein is stable for 12 months at -80 °C, for 2-4 weeks at 4°C.
Suggested Usage:	Useful for studying enzyme kinetics, screening for PKM2 modulators including activators and inhibitors, and selectivity profiling. Protein binding assay, antigen, ELSA, Western blot, crystallization, co-crystallization and crystal structure determination study.