For immobilization of DNA with a surface epoxy groups. Purification of a transcription factor and complementary DNA or RNA is one of applications.

 

Storage buffer	ultrapure water
Magnetization	superparamagnetism (>10 emu/g)
Size of beads	180 nm±30 nm
Concentration	20 mg/ml
Functional groups	Epoxy groups
Amounts of the functional groups	Approx. 1μmol/mg

>> For more information, please visit here.

 

It is difficult for the OH groups to immobilize it to beads in reactivity being low, but phenol groups can be immobilized on epoxy beads under an alkaline condition because reactivity is high. Because epoxy beads produce almost no non-specific protein adsorption, it is not necessary to mask the remaining epoxy groups.

 

Storage buffer	ultrapure water
Magnetization	superparamagnetism (>10 emu/g)
Size of beads	180 nm±30 nm
Concentration	20 mg/ml
Functional groups	Epoxy groups
Amounts of the functional groups	200-300 nmol/mg

>> For more information, please visit here.

Compounds with COOH groups are immobilized on NH₂ beads by covalent bond. But it is necessary to let a ligand activate with NHS ester.

 

Storage buffer	ultrapure water
Magnetization	superparamagnetism (>10 emu/g)
Size of beads	180 nm±30 nm
Concentration	20 mg/ml
Functional groups	NH2 groups
Amounts of the functional groups	200-300 nmol/mg

>> For more information, please visit here.

 

When the reactivity of the COOH groups is high, compounds with COOH groups are immobilized on OH beads by covalent bond. Because OH beads produce almost no non-specific protein adsorption, it is not necessary to mask the remaining OH groups.

 

Storage buffer	ultrapure water
Magnetization	superparamagnetism (>10 emu/g)
Size of beads	180 nm±30 nm
Concentration	20 mg/ml
Functional groups	OH groups
Amounts of the functional groups	200-300 nmol/mg

>> For more information, please visit here.

Compounds with NH₂ groups are immobilized on COOH beads by covalent bond easily. Therefore we can purify target protein at high purity from samples such as the cell extract. And, because antibodies or proteins with NH₂ groups are immobilized easily, we can use it for immunoprecipitation and cell separation.

 

Storage buffer	isopropyl alcohol
Magnetization	superparamagnetism (>10 emu/g)
Size of beads	180 nm±30 nm
Concentration	20 mg/ml
Functional groups	NHS groups
Amounts of the functional groups	Approx. 1μmol/mg

>> For more information, please visit here.

 

Compounds, antibodies and proteins with NH₂ groups are immobilized on COOH beads by covalent bond. We can use it in wide application such as the chemical biology or cell separation.

 

Storage buffer	ultrapure water
Magnetization	superparamagnetism (>10 emu/g)
Size of beads	180 nm±30 nm
Concentration	20 mg/ml
Functional groups	COOH groups
Amounts of the functional groups	200-300 nmol/mg

>> For more information, please visit here.

Compounds with alkyne are immobilized on azide beads easily by using a click chemistry reaction. When a compound has plural one functional group, we can immobilize the compound which we introduced an alkyne into on azide beads regiospecifically.

 

Storage buffer	ultrapure water
Magnetization	superparamagnetism (>10 emu/g)
Size of beads	180 nm±30 nm
Concentration	20 mg/ml
Functional groups	Azide groups
Amounts of the functional groups	70-140 nmol/mg

>> For more information, please visit here.

 

Compounds with azide groups are immobilized on alkyne beads easily by using a click chemistry reaction. When a compound has plural one functional group, we can immobilize the compound which we introduced an azide group into on alkyne beads regiospecifically.

 

Storage buffer	ultrapure water
Magnetization	superparamagnetism (>10 emu/g)
Size of beads	180 nm±30 nm
Concentration	20 mg/ml
Functional groups	Alkyne groups
Amounts of the functional groups	70-140 nmol/mg

>> For more information, please visit here.

His tagged proteins can be immobilized to Ts beads selectively. This shows superiority for elucidation of the protein-protein interaction.

 

Storage buffer	1mM HCl
Magnetization	superparamagnetism (>10 emu/g)
Size of beads	180 nm±30 nm
Concentration	20 mg/ml
Functional groups	Ts groups
Amounts of the functional groups	200-300 nmol/mg

>> For more information, please visit here.

Protein A beads are combined with antibody (IgG) mainly. We can use it for the immunoprecipitation experiment using an antigen - antibody reaction or the antibody purification.

 

Storage buffer	10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10% glycerol
Magnetization	superparamagnetism (>10 emu/g)
Size of beads	180 nm±30 nm
Concentration	20 mg/ml
Functional groups	Protein A
Capacity	>35 ug Human IgG/mg of beads

>> For more information, please visit here.

 

Protein G beads are combined with antibody (IgG) mainly. We can use it for the immunoprecipitation experiment using an antigen - antibody reaction or the antibody purification.

 

Storage buffer	10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10%glycerol
Magnetization	superparamagnetism (>10 emu/g)
Size of beads	180 nm±30 nm
Concentration	20 mg/ml
Functional groups	Protein G
Capacity	>35 ug Human IgG/mg of beads

>> For more information, please visit here.

  

Biotinylated compounds and biotin-labeled antibody or DNA can be immobolized on Streptavidin beads, and we can use it in wide application. In addition, we can use Streptavidin beads for the separation by biotin-labeled cell.

 

Storage buffer	10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10%glycerol
Magnetization	superparamagnetism (>10 emu/g)
Size of beads	180 nm±30 nm
Concentration	20 mg/ml
Functional groups	Streptavidin
Capacity	>3 ug biotinylated BSA/mg of beads

>> For more information, please visit here.

 

Biotinylated compounds and biotin-labeled antibody or DNA can be immobolized on NeutrAvidin beads, and we can use it in wide application.

 

Storage buffer	10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10%glycerol
Magnetization	superparamagnetism (>10 emu/g)
Size of beads	180 nm±30 nm
Concentration	20 mg/ml
Functional groups	NeutrAvidin
Capacity	>1.5 ug biotinylated BSA/mg of beads

>> For more information, please visit here.