Nacalai USA - Innovations for Life Sciences

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FG beads®: Line-up

Plain beads / Linker beads (Epoxy beads)

  • Plain beads
For immobilization of DNA with a surface epoxy groups. Purification of a transcription factor and complementary DNA or RNA is one of applications.
 
Storage buffer ultrapure water
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Epoxy groups
Amounts of the functional groups Approx. 1μmol/mg

>> For more information, please visit here.

 

  • Linker beads (Epoxy beads)
It is difficult for the OH groups to immobilize it to beads in reactivity being low, but phenol groups can be immobilized on epoxy beads under an alkaline condition because reactivity is high. Because epoxy beads produce almost no non-specific protein adsorption, it is not necessary to mask the remaining epoxy groups.
 
Storage buffer ultrapure water
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Epoxy groups
Amounts of the functional groups 200-300 nmol/mg

>> For more information, please visit here.

NH2 beads / OH beads

  • NH2 beads
Compounds with COOH groups are immobilized on NH2 beads by covalent bond. But it is necessary to let a ligand activate with NHS ester.
 
Storage buffer ultrapure water
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups NH2 groups
Amounts of the functional groups 200-300 nmol/mg

>> For more information, please visit here.

 

  • OH beads
When the reactivity of the COOH groups is high, compounds with COOH groups are immobilized on OH beads by covalent bond. Because OH beads produce almost no non-specific protein adsorption, it is not necessary to mask the remaining OH groups.
 
Storage buffer ultrapure water
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups OH groups
Amounts of the functional groups 200-300 nmol/mg

>> For more information, please visit here.

NHS beads / COOH beads

  • NHS beads
Compounds with NH2 groups are immobilized on COOH beads by covalent bond easily. Therefore we can purify target protein at high purity from samples such as the cell extract. And, because antibodies or proteins with NH2 groups are immobilized easily, we can use it for immunoprecipitation and cell separation.
 
Storage buffer isopropyl alcohol
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups NHS groups
Amounts of the functional groups Approx. 1μmol/mg

>> For more information, please visit here.

 

  • COOH beads
Compounds, antibodies and proteins with NH2 groups are immobilized on COOH beads by covalent bond. We can use it in wide application such as the chemical biology or cell separation.
 
Storage buffer ultrapure water
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups COOH groups
Amounts of the functional groups 200-300 nmol/mg

>> For more information, please visit here.

Azide beads / Alkyne beads

  • Azide beads
Compounds with alkyne are immobilized on azide beads easily by using a click chemistry reaction. When a compound has plural one functional group, we can immobilize the compound which we introduced an alkyne into on azide beads regiospecifically.
 
Storage buffer ultrapure water
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Azide groups
Amounts of the functional groups 70-140 nmol/mg

>> For more information, please visit here.

 

  • Alkyne beads
Compounds with azide groups are immobilized on alkyne beads easily by using a click chemistry reaction. When a compound has plural one functional group, we can immobilize the compound which we introduced an azide group into on alkyne beads regiospecifically.
 
Storage buffer ultrapure water
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Alkyne groups
Amounts of the functional groups 70-140 nmol/mg

>> For more information, please visit here.

Ts beads

  • Ts beads
His tagged proteins can be immobilized to Ts beads selectively. This shows superiority for elucidation of the protein-protein interaction.
 
Storage buffer 1mM HCl
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Ts groups
Amounts of the functional groups 200-300 nmol/mg

>> For more information, please visit here.

Protein A beads / Protein G beads / Streptavidin beads / NeutrAvidin beads

  • Protein A beads
Protein A beads are combined with antibody (IgG) mainly. We can use it for the immunoprecipitation experiment using an antigen - antibody reaction or the antibody purification.
 
Storage buffer 10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10% glycerol
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Protein A
Capacity >35 ug Human IgG/mg of beads

>> For more information, please visit here.

 

  • Protein G beads
Protein G beads are combined with antibody (IgG) mainly. We can use it for the immunoprecipitation experiment using an antigen - antibody reaction or the antibody purification.
 
Storage buffer 10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10%glycerol
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Protein G
Capacity >35 ug Human IgG/mg of beads

>> For more information, please visit here.

  

  • Streptavidin beads
Biotinylated compounds and biotin-labeled antibody or DNA can be immobolized on Streptavidin beads, and we can use it in wide application. In addition, we can use Streptavidin beads for the separation by biotin-labeled cell.
 
Storage buffer 10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10%glycerol
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups Streptavidin
Capacity >3 ug biotinylated BSA/mg of beads

>> For more information, please visit here.

 

  • NeutrAvidin beads
Biotinylated compounds and biotin-labeled antibody or DNA can be immobolized on NeutrAvidin beads, and we can use it in wide application.
 
Storage buffer 10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10%glycerol
Magnetization superparamagnetism (>10 emu/g)
Size of beads 180 nm±30 nm
Concentration 20 mg/ml
Functional groups NeutrAvidin
Capacity >1.5 ug biotinylated BSA/mg of beads

>> For more information, please visit here.

Ordering Information

Product Storage Cat.No. PKG Size Price  
Plain beads 4°C TAS8848N1010-10MG 10 mg 247.00
Buy
Plain beads 4°C TAS8848N1010-20MG 20 mg 274.00
Buy
Linker beads (Epoxy beads) 4°C TAS8848N1110-5MG 5 mg 164.00
Buy
Linker beads (Epoxy beads) 4°C TAS8848N1110-20MG 20 mg 420.00
Buy
OH beads 4°C TAS8848N1120-5MG 5 mg 240.00
Buy
OH beads 4°C TAS8848N1120-20MG 20 mg 555.00
Buy
NH2 beads 4°C TAS8848N1130-5MG 5 mg 240.00
Buy
NH2 beads 4°C TAS8848N1130-20MG 20 mg 555.00
Buy
COOH beads 4°C TAS8848N1140-5MG 5 mg 274.00
Buy
COOH beads 4°C TAS8848N1140-20MG 20 mg 683.00
Buy
NHS beads -20°C TAS8848N1141-5MG 5 mg 342.00
Buy
NHS beads -20°C TAS8848N1141-10MG 10 mg 547.00
Buy
NHS beads -20°C TAS8848N1141-20MG 20 mg 956.00
Buy
Ts beads 4°C TAS8848N1150-5MG 5 mg 274.00
Buy
Ts beads 4°C TAS8848N1150-20MG 20 mg 683.00
Buy
Azide beads 4°C TAS8848N1160-5MG 5 mg 479.00
Buy
Azide beads 4°C TAS8848N1160-10MG 10 mg 683.00
Buy
Azide beads 4°C TAS8848N1160-20MG 20 mg 1,092.00
Buy
Alkyne beads 4°C TAS8848N1161-5MG 5 mg 479.00
Buy
Alkyne beads 4°C TAS8848N1161-10MG 10 mg 683.00
Buy
Alkyne beads 4°C TAS8848N1161-20MG 20 mg 1,092.00
Buy
HM-Streptavidin beads 4°C TAB8848N3170-5MG 5 mg 956.00
Buy
HM-Streptavidin beads 4°C TAB8848N3170-10MG 10 mg 1,366.00
Buy
HM-Streptavidin beads 4°C TAB8848N3170-20MG 20 mg 2,184.00
Buy
HM-NeutrAvidin beads 4°C TAB8848N3171-5MG 5 mg 956.00
Buy
HM-NeutrAvidin beads 4°C TAB8848N3171-10MG 10 mg 1,366.00
Buy
HM-NeutrAvidin beads 4°C TAB8848N3171-20MG 20 mg 2,184.00
Buy
HM-Protein A beads 4°C TAB8848N3172-5MG 5 mg 956.00
Buy
HM-Protein A beads 4°C TAB8848N3172-20MG 20 mg 1,366.00
Buy
HM-Protein A beads 4°C TAB8848N3172-100MG 100 mg 2,184.00
Buy
HM-Protein G beads 4°C TAB8848N3173-5MG 5 mg 956.00
Buy
HM-Protein G beads 4°C TAB8848N3173-20MG 20 mg 1,366.00
Buy
HM-Protein G beads 4°C TAB8848N3173-100MG 100 mg 2,184.00
Buy
Streptavidin beads 4°C TAS8848N1170-5MG 5mg 479.00
Buy
Streptavidin beads 4°C TAS8848N1170-10MG 10mg 683.00
Buy
Streptavidin beads 4°C TAS8848N1170-20MG 20mg 1,092.00
Buy
NeutrAvidin beads 4°C TAS8848N1171-5MG 5mg 479.00
Buy
NeutrAvidin beads 4°C TAS8848N1171-10MG 10mg 683.00
Buy
NeutrAvidin beads 4°C TAS8848N1171-20MG 20mg 1,092.00
Buy
Protein A beads 4°C TAS8848N1172-5MG 5mg 479.00
Buy
Protein A beads 4°C TAS8848N1172-10MG 10mg 683.00
Buy
Protein A beads 4°C TAS8848N1172-20MG 20mg 1,092.00
Buy
Protein G beads 4°C TAS8848N1173-5MG 5mg 479.00
Buy
Protein G beads 4°C TAS8848N1173-10MG 10mg 683.00
Buy
Protein G beads 4°C TAS8848N1173-20MG 20mg 1,092.00
Buy

Storage buffer of HM beads: 10 mM HEPES (pH 7.9)