Protocols & FAQ
Protocols
FAQ
- Are the sizes of FG beads® all equal?
The sizes are 0.2 μm. The CV value indicating variation is approximately 30%.
- What is the linker length of FG beads®?
It is about 1 nm.
- What is the number of particles per 1 mg of FG beads®?
It is about 1.8×1011 particles.
- What types of the FＧ beads are there?
At present, 13 types of FG beads® are available, with different surface modificationssuch as functional groups or bound proteins. Additions to the lineup are currently being considered.
In addition to FG beads®, there are also fluorescent beads. - What is the approximate quantity of the functional groups on FG beads®?
The amount of epoxy on Plain beads is 1 μmol per 1 mg of beads. The amount of functional groups on COOH beads and NHS beads is 200 – 300 nmol per 1 mg of beads.
- Are the FG beads® resistant to heat?
There are no problems up to approximately 70°C. However the type of beads having high functional reactivity of the linker end may come under an influence by the heat.
- Are the FG beads® resistant to pH?
There are no problems within the range of pH2 - pH11. However the protein immobilized on beads may denature and the performance may decrease.
- Is there the resistance to organic solvents of FG beads® in addition to DMF?
Ligands can be immobilized on the surfaces of FG beads® in various organic solvents, such as methanol, DMF, DMSO, THF, ethyl acetate, pyridine, dioxane, toluene, dichloromethane, chloroform, etc.
- Can the FG beads® be reused?
It is believed that the beads can be reused when salt elution or drug elution was performed. However boil elution causes degeneration of the FG beads® and makes it impossible for them to be reused.
- How should one select a bead type?
Select the FG beads® with the optimal function group according to the substance or protein that you will bind, or select streptavidin beads