COSMOSIL SFC Column Series
| Supercritical Fluid Chromatography (SFC) has become more attractive because it offers some advantages over HPLC, such as high speed, unique selectivity and environmentally friendly separations. Many conventional normal-phase stationary phases, such as diol, amino and cyano, have been used for SFC applications. However, these phases present limitations for separations. COSMOSIL SFC Columns have been developed to enhance the capability of SFC separations. |  |
Features
- Three categories (I–III) of stationary phase for different types of compounds
- Different selectivity from HPLC
Product Information
Category I: Columns for mid- to high-polarity compounds
For these compounds, a high-polarity stationary phase is suitable. More polar compounds are retained longer.
| Product | Stationary phase | Features |
| COSMOSIL PY | Pyridinyl | Similar selectivity to 2-ethylpyridine; strong retention in general. |
| COSMOSIL HP | 3-Hydroxyphenyl | Different selectivity from PY; strong retention for basic compounds. |
| COSMOSIL Diol | Diol | Less effect from ionic interaction. |
Category II: Columns for low-polarity compounds
For these compounds, a low-polarity stationary phase is suitable.
| Product | Stationary phase | Features |
| COSMOSIL Cholester | Cholesteryl | Longer retention and better separation than C18. |
Category III: Columns for SFC-specific separations
In supercritical fluid chromatography (SFC), secondary interactions such as π-π and dispersion force* are stronger compared to high-performance liquid chromatography (HPLC). As a result, these columns are capable of unique separations in SFC.
| Product | Stationary phase | Features |
| COSMOSIL πMAX | Pyrenylethyl | Stronger π-π interactions than phenyl columns. |
| COSMOSIL PBr | Pentabromobenzyl | Unique separations using dispersion force.* |
* Dispersion force:
London dispersion force is a weak intermolecular force that results from dipoles temporarily induced from random unsymmetrical electron positions in two adjacent atoms, also known as "instantaneous dipole-induced dipole force". It is present in all molecules, regardless of whether they are polar or non-polar. Compounds with high polarizability have stronger dispersion force.
Packing Material Properties
| Packing material | PY | HP | Diol | Cholester | πMAX | PBr |
|---|---|---|---|---|---|---|
| Silica gel | High-purity porous spherical silica | |||||
| Average particle size | 3, 5 µm | 5 µm | 5 µm | 5 µm | 5 µm | |
| Average pore size | 120Å | |||||
| Stationary phase |  |
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| Pyridinyl | 3-Hydroxyphenyl | Diol | Cholesteryl | Pyrenylethyl | Pentabromobenzyl | |
| Endcapping treatment | Near-Perfect Treatment | |||||
For information on pricing of COSMOSIL Cholester and PBr, please visit the following website;
>> COSMOSIL PBr
Selection by Polarity
Derivatives of xanthine (high-polarity compounds) XLogP3: -0.8 – 0.0
Category I columns (COSMOSIL 5PY, 5HP, 5Diol) and COSMOSIL 5πMAX separated the sample well. The elution order with COSMOSIL 5πMAX was different than the category I columns.
| Column Size | 4.6 mmI.D. x 250 mm |
| Mobile Phase | A: CO2 B: Methanol B conc. 10% (πMAX only: 20%) |
| Flow Rate | 3.0 ml/min |
| BPR | 10 MPa |
| Temperature | 40 oC |
| Detection | UV 273 nm |
| Sample |
1. Caffeine (0.10 mg/mL)
2. Theophylline (0.10 mg/mL)
3. Theobromine (0.09 mg/mL)
|
| Inj. Vol. |
5.0 µl
|
Positional isomers (mid-polarity compounds) XLogP3: 1.4 – 1.9
Category I columns (COSMOSIL 5PY, 5HP, 5Diol) yielded the best separation.
| Column Size | 4.6 mmI.D. x 250 mm |
| Mobile Phase | A: CO2 B: Methanol B conc. 5% |
| Flow Rate | 3.0 ml/min |
| BPR | 10 MPa |
| Temperature | 40 oC |
| Detection | UV 254 nm |
| Sample |
1. o-Hydroxyacetophenone (0.1 mg/mL)
2. m-Hydroxyacetophenone (0.1 mg/mL)
3. p-Hydroxyacetophenone (0.1 mg/mL)
|
| Inj. Vol. |
5.0 µl
|
Positional isomers (low-polarity compounds) XLogP3: 5.8 – 6.0
Category III (COSMOSIL 5πMAX, 5PBr) and category II (5Cholester) columns separated most effectively.
| Column Size | 4.6 mmI.D. x 250 mm |
| Mobile Phase | A: CO2 B: Methanol B conc. 5% (PBr only: 15%) |
| Flow Rate | 3.0 ml/min |
| BPR | 10 MPa |
| Temperature | 40 oC |
| Detection | UV 254 nm |
| Sample |
1. o-Terphenyl(0.1 mg/mL)
2. m-Terphenyl(0.1 mg/mL)
3. p-Terphenyl(0.1 mg/mL)
|
| Inj. Vol. |
5.0 µl
|
Column Comparison Data
Amitriptyline
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Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics
Reserpine
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Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics
3-Aminobenzoic Acid
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Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics
Benzoic Acid
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Comparison of Retention Behavior
The following three stationary phases were evaluated for their retention of hydrophilic, hydrophobic, acidic and basic compounds. COSMOSIL HP and PY elute hydrophobic compounds first and retain hydrophilic compounds longer, whereas PBr elutes in the reverse order, exhibiting high retention for hydrophobic compounds. HP had the longest retention for basic compounds.
| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | A: CO2 B: 0.1% CH3COONH4 - Methanol B conc. 0→50% (0→14min), 50% (14-17min) |
| Flow rate: | 0.8 ml/min |
| BPR | 10 MPa |
| Temperature: | 40°C |
| Detection: | ESI-MS/MS, MRM |
| Sample |
(No.1) Uridine |
| Sample conc. | 8 µmol/l |
| Inj. Vol | 2.0 µl |
Applications
Vitamin D
COSMOSIL 5πMAX and 5PBr were able to separate the vitamins. The PFP (pentafluorophenyl) column, which also uses a halogenated stationary phase, could not separate them. The dispersion force used by PBr interacts more strongly with larger molecules. PFP, which is a smaller molecule, does not exhibit this selectivity.
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Column Size
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4.6 mmI.D. x 250 mm
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Mobile Phase
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A: CO2 B: Methanol B conc. **%
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Flow Rate
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3.0 ml/min
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BPR
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10 MPa
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Temperature
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40 oC
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Detection
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UV 265 nm
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Sample
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1. Vitamin D3 (0.6 mg/mL)
2. Vitamin D2 (0.6 mg/mL)
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Inj. Vol.
|
3.0 µl
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Vitamin D2/D3
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Column
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COSMOSIL πMAX
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Column Size
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4.6 mmI.D. x 150 mm
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Mobile Phase
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60% CO2: 40% MeOH in 5 min |
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Flow Rate
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3.0 ml/min
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BPR
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160 bar
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Temperature
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25 oC
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Detection
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UV 265 nm
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Inj. Vol.
|
2.0 µl
|
Data courtesy of Pfizer Inc. La Jolla, California
Nucleobases
COSMOSIL 5Diol separated 5 nucleic acid bases.
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Column Size
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4.6 mmI.D. x 250 mm
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Mobile Phase
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A: CO2 B: 100mmol/l Ammounium Acetate-Methanol B conc. **%
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Flow Rate
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3.0 ml/min
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BPR
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10 MPa
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Temperature
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40 oC
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Detection
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UV 260 nm
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Sample
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Inj. Vol.
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5.0 µl
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Polystyrene
These polystyrene samples with different degrees of polymerization are likely separated by number of monomer units. COSMOSIL 5πMAX and 5PBr were able to separate the high-MW polystyrene.
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Column Size
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4.6 mmI.D. x 250 mm
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Mobile Phase
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A: CO2 B: tetrahydrpfuran B conc. *→*% 10min Linear gradient
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Flow Rate
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3.0 ml/min
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BPR
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10 MPa
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Temperature
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40 oC
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Detection
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UV 220 nm
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Sample
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Polystyrene, MW906 (5.0 mg/mL)
Polystyrene, MW2200 (5.0 mg/mL)
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Inj. Vol.
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5.0 µl
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Polyaromatic Compounds
Fat-Soluble Vitamins
COSMOSIL Cholester exhibits strong retention for fat-soluble vitamins and is suitable for on-line SFE-SFC using Shimadzu’s Nexera UC. The on-line extraction from food also produced triglyceride impurities, which were successfully separated from the vitamins.
Surfactant
Non-steroidal anti-inflammatory drugs
| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | Carbon Dioxide : Methanol |
| Gradient: | 95:5 to 65:35 over 6 minutes |
| Flow rate: | 5.0 ml/min |
| Temperature: | 30°C |
| Detection: | UV230nm |
| Sample: | 1: Ibuprofen 2: Fenoprofen 3: Flurbiprofen 4: Ketoprofen 5: Indoprofen |
Beta Blockers
Peak elution order reversal under identical Conditions
| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | Carbon Dioxide : Methanol w/20mM ammonium formate |
| Gradient: | 95:5 to 50:50 over 4 minutes |
| Flow rate: | 5.0 ml/min |
| Temperature: | 30°C |
| Detection: | APCI(+) |
| Sample: | 1. Pindolol 2. Atenolol |
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Applications -2
Polyethylene Glycols (PEGs)
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Methyl Mandelate
Polyaromatic Compounds
Catecholamines
Angiotensin
Downloads
| Brochure | COSMOSIL SFC Columns |  (1.2 MB) |
| Technical Guide | COSMOSIL SFC Technical Guide | (1 MB) |
| Posters |
Research into the Retention Mechanism of SFC
(Poster from SFC Asia 2017, Osaka, Japan)
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Development of novel stationary phases for Supercritical Fluid Chromatography (SFC) (Poster from HPLC 2011, Budapest, Hungary)
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COSMOSIL Application Search
COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size
Ordering Information
COSMOSIL πMAX
| Product | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|
| COSMOSIL 3πMAX Packed Column (2.1 mmI.D. x 150 mm) | 19596-11 | 1 pkg | 1,049.00 | Buy |
| COSMOSIL 3πMAX Packed Column (4.6 mmI.D. x 250 mm) | Inquiry | 1 pkg | - | Inquire |
| COSMOSIL 5πMAX Packed Column (2.1 mmI.D. x 150 mm) | 18696-31 | 1 pkg | 1,006.00 | Buy |
| COSMOSIL 5πMAX Packed Column (4.6 mmI.D. x 250 mm) | 18372-61 | 1 pkg | 1,006.00 | Buy |
| COSMOSIL 5πMAX Guard Column (10.0 mmI.D. x 20 mm) | 18867-91 | 1 pkg | 1,434.00 | Buy |
| COSMOSIL 5πMAX Packed Column (10.0 mmI.D. x 250 mm) | 18679-61 | 1 pkg | 2,547.00 | Buy |
| COSMOSIL 5πMAX Packed Column (20.0 mmI.D. x 250 mm) | 19587-31 | 1 pkg | 5,918.00 | Buy |
COSMOSIL Diol
| Product | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|
| COSMOSIL 3Diol Packed Column (2.1 mmI.D. x 150 mm) | 21567-21 | 1 pkg | 1,092.00 | Buy |
| COSMOSIL 5Diol Packed Column (2.1 mmI.D. x 150 mm) | 18253-21 | 1 pkg | 1,006.00 | Buy |
| COSMOSIL 5Diol Packed Column (4.6 mmI.D. x 250 mm) | 18252-31 | 1 pkg | 1,006.00 | Buy |
| COSMOSIL 5Diol Packed Column (10.0 mmI.D. x 250 mm) | 18678-71 | 1 pkg | 2,344.00 | Buy |
| COSMOSIL 5Diol Packed Column (20.0 mmI.D. x 250 mm) | 18869-71 | 1 pkg | - | Inquire |
COSMOSIL HP (3-Hydroxyphenyl)
| Product | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|
| COSMOSIL 3HP Packed Column (2.1 mm I.D. x 150 mm) | 13559-81 | 1 pkg | 889.00 | Buy |
| COSMOSIL 3HP Packed Column (4.6 mm I.D. x 250 mm) | 13560-41 | 1 pkg | 942.00 | Buy |
| COSMOSIL 5HP Packed Column (2.1 mm I.D. x 150 mm) | 13845-21 | 1 pkg | 782.00 | Buy |
| COSMOSIL 5HP Packed Column (4.6 mm I.D. x 100 mm) | 13971-61 | 1 pkg | 656.00 | Buy |
| COSMOSIL 5HP Packed Column (4.6 mm I.D. x 150 mm) | 13972-51 | 1 pkg | 656.00 | Buy |
| COSMOSIL 5HP Packed Column (4.6 mm I.D. x 250 mm) | 13854-01 | 1 pkg | 835.00 | Buy |
| COSMOSIL 5HP Guard Column (10.0 mm I.D. x 20 mm) | 13777-21 | 1 pkg | 1,252.00 | Buy |
| COSMOSIL 5HP Packed Column (10.0 mm I.D. x 150 mm) | 13973-41 | 1 pkg | 1,424.00 | Buy |
| COSMOSIL 5HP Packed Column (10.0 mm I.D. x 250 mm) | 13776-31 | 1 pkg | 1,922.00 | Buy |
| COSMOSIL 5HP Packed Column (20.0 mm I.D. x 150 mm) | 13855-91 | 1 pkg | 5,136.00 | Buy |
| COSMOSIL 5HP Packed Column (20.0 mm I.D. x 250 mm) | 13778-11 | 1 pkg | 6,848.00 | Buy |
COSMOSIL PY (Pyridinyl)
| Product | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|
| COSMOSIL 3PY Packed Column (2.1 mmI.D. x 150 mm) | 13557-01 | 1 pkg | 889.00 | Buy |
| COSMOSIL 3PY Packed Column (4.6 mmI.D. x 250 mm) | 13558-91 | 1 pkg | 942.00 | Buy |
| COSMOSIL 5PY Packed Column (2.1 mmI.D. x 150 mm) | 13844-31 | 1 pkg | 717.00 | Buy |
| COSMOSIL 5PY Packed Column (4.6 mmI.D. x 100 mm) | 13974-31 | 1 pkg | 656.00 | Buy |
| COSMOSIL 5PY Packed Column (4.6 mmI.D. x 150 mm) | 13975-21 | 1 pkg | 2,758.00 | Buy |
| COSMOSIL 5PY Packed Column (4.6 mmI.D. x 250 mm) | 13846-11 | 1 pkg | 696.00 | Buy |
| COSMOSIL 5PY Guard Column (10.0 mmI.D. x 20 mm) | 13781-51 | 1 pkg | 1,252.00 | Buy |
| COSMOSIL 5PY Packed Column (10.0 mmI.D. x 150 mm) | 13976-11 | 1 pkg | 1,421.00 | Buy |
| COSMOSIL 5PY Packed Column (10.0 mmI.D. x 250 mm) | 13780-61 | 1 pkg | 1,922.00 | Buy |
| COSMOSIL 5PY Packed Column (20.0 mmI.D. x 150 mm) | 13977-01 | 1 pkg | 3,343.00 | Buy |
| COSMOSIL 5PY Packed Column (20.0 mmI.D. x 250 mm) | 13782-41 | 1 pkg | 4,429.00 | Buy |