Malic Enzymes catalyze the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as cofactors. These proteins have a wide variety of uses including but not limited to; Protein binding Assays, Small Molecule Inhibitor Screens,Western blotting, ELISA, Crystallization and Co-Crystallization studies, Kinase substrates, and human antigen studies.

Recombinant Human ME1

ME1 is a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

 

Synonym(s):	NADP-dependent malic enzyme, Malic enzyme 1
Molecular Weight :	65.2 kDa
Tag :	His tag C-terminus
Source :	E. coli
Purity :	Greater than 95%, as determined by SDS-PAGE
Formulation :	30 mM Tris pH 7.4, 500 mM NaCl, 10% glycerol, 3 mM MgCl2, 5 mM beta-mercaptoethanol, 1 mM PMSF, 1 mM Benzamidine
Reconstitution :	Clear solution in 30 mM Tris, pH7.4, 150 mM NaCl, 40% Glycerol, 5 mM DTT
Application :	Binding assay, small molecule inhibitor screening, substrate of kinase, antigen, ELISA, Western blot, crystallization, and co-crystallization studies.
Storage :	Upon delivery aliquot and store at -80 °C. Avoid multiple freeze-thaw cycles. The protein is stable for 12 months at -80 °C, for 2-4 weeks at 4°C.
Suggested Usage :	Protein binding assay, small molecule inhibitor screening, substrate of kinase, antigen, ELISA, Western blot, crystallization and co- crystallization studies.