SUMO Protease
Tag Removal Protease / Recombinant SUMO Protease (Ready to Use)
| SUMO Protease otherwise known as Ulp1, is a recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae. This highly specific protease cleaves off sumo tags by recognizing the tertiary structure of SUMO as opposed to recognizing its amino acid sequence. Because of its recognition of SUMO’s tertiary structure, it has little to no non-specific proteolysis keeping your protein samples safe after all affinity tags have been cleaved off. SUMO protease is active from 2°C to 37°C in a pH range of 7.0 – 9.0, for lower temperatures allow more time for the cleavage reaction. |
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Synonym(s) :
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ULP1, Ulp, Ubl-specific protease 1
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Molecular Weight:
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27.4 kDa
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Purity :
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Greater than 90% by SDS-PAGE
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Tag :
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N-terminal His-tag
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Source:
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E. coli
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GeneBank Accession # :
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QHB12236.1 |
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Activity :
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≥3,000 units/mg protein
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Unit Definition :
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One unit of SUMO protease cleaves >85% of 3 µg of control substrate in one hour at pH 8.0 at 30 °C.
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Application ;
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Recombinant protein SUMO tag removal, recombinant protein characterization, ELISA, Western blot, crystallization studies.
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Formulation :
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20 mM Tris-HCl, pH8.0, 350 mM NaCl, 10% Glycerol, 1mM 2-Mercaptoethanol
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Storage :
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Stable for 12 months at -80°C, Avoid freeze/thaw cycles
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| Reference : | Elmore, Z.C., et al., BMC Biology 9, 74 (2011). |