The Prestained Protein Ladder, Mid Range is a combination of 10 pre-stained proteins with molecular weights from 11 to 180 kDa. The 10 recombinant proteins are covalently coupled with blue chromophore, while a red band at 80 kDa, a green band at 25 kDa, and a newly designed yellow band at 15 kDa serve as reference bands. The Prestained Protein Ladder, Mid Range keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

Guide for Molecular Weight Estimation (kDa)

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Storage Buffer

0.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.

Quality Control

5 μl of Prestained Protein Ladder resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.

Storage

Stable for up to 2 weeks at 25°C
Stable for up to 3 months at 4°C
Stable for up to 24 months at -20°C