| Product Name | Chemi-Lumi One L | Chemi-Lumi One Super | Chemi-Lumi One Ultra |
|---|---|---|---|
| Product No. | 07880-70 | 02230-30 | 11644-40 |
| Usage | General Use | High Sensitive | Ultra high Sensitive |
| Lower Detection Limit | Low-picogram | Mid-femtogram | Low-femtogram |
| Required Working Solution | approx. 0.125 ml / cm2 | approx. 0.1 ml / cm2 | approx. 0.1 ml / cm2 |
| Suggested Antibody Dilution | Primary: 1:1,000-1:5,000 Secondary: 1:20,000-1:100,000 |
Primary: 1:1,000-1:20,000 Secondary: 1:20,000-1:200,000 |
Primary: 1:5,000-1:100,000 Secondary: 1:100,000-1:500,000 |
| Reaction Period | 1 min. | 1 min. | 5 min. |
| Comparable to | Amersham TM ECLTM SuperSignal® West Pico® |
Amersham TM ECLTM Prime SuperSignal® West Dura® |
Amersham TM ECLTM Select SuperSignal® West Femto® |
| Sensitivity |
<Condition> |
||
Chemiluminescent HRP Substrates : Chemi-Lumi One Ultra
Low-femtogram Sensitivity Substrate for HRP / The Highest Sensitivity
Features
- Low-femtogram sensitivity
- Longer signal duration
Chemi-Lumi One Series for HRP
Chemi-Lumi One is a series of high sensitive luminol-based chemiluminescence assay kits for Western Blotting. We offer three types of chemiluminescent substrates for Western blot detection with horseradish peroxidase enzyme (HRP).
Comparison of Sensitivity with SuperSigmal West Femto
Chemi-Lumi One Ultra is an extremely sensitive chemiluminescent substrate. Western blot of identical transferrin samples. The membranes were incubated with substrate that was prepared according to the manufacturers' instructions. The membranes were exposed to film for 2 minutes.
- Primary antibody : Rabbit anti-transferrin, 1: 30,000
- Secondary antibody: Goat anti-rabbit IgG-HRP, 1: 200,000
- Primary antibody : Rabbit anti-transferrin, 1: 30,000
- Secondary antibody: Goat anti-rabbit IgG-HRP, 1: 200,000
Comparison of Sensitivity with Competitors
Chemi-Lumi One Ultra shows equal to or higher sensitivity compared to competitors'
| Gel: | 10% PAGE gel |
| Wash: | 0.1% t-TBS (x1), pH 7.4 |
| Blocking: | Blocking One (Cat. No.: 03953) |
| 1st Ab: | Anti-β-Actin Mouse Monoclonal Antibody (Santa Cruz, Cat. No.: sc-47778), 1:300 |
| 2nd Ab: | Anti-Mouse IgG (Goat), HRP Conjugated (Santa Crus, Cat. No.: sc-2005), 1: 1000 |
| Detection Period: | 5 min. |
| Detector: | LAS-3000 High Mode |
| Expose Time: | 3 min. |
Comparison of Abs Concentration
Due to low background, Chemi-Lumi One Ultra is applicable to wide range of primary antibodies, and makes it easy to optimize condition.
| Gel: | 10% PAGE gel |
| Wash: | 0.1% t-TBS (x1), pH 7.4 |
| Blocking: | Blocking One (Cat. No.: 03953) |
| 1st Ab: | Anti-PCNA Mouse Monoclonal Antibody (Santa Cruz, Cat. No.: sc-56c) |
| 2nd Ab: | Anti-Mouse IgG (Goat), HRP Conjugated (Santa Crus, Cat. No.: sc-2005), 1: 100,000 |
| Detection Period: | 5 min. |
| Detector: | LAS-3000 High Mode |
| Expose Time: | 1:1000, 3 min. / 1:5000, 5 min. / 1:10,000, 8 min. |
Chemi-Lumi One Ultra (Instruction)Reference
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Iwasaki, Takeshi, et al. "Establishment and Characterization of a Novel Primitive Yolk Sac Tumour Cell Line, TC587." Anticancer research 40.2 (2020): 759-766.
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Saito, Akatsuki, et al. "The 4th and 112th residues of viral capsid cooperatively modulate capsid-CPSF6 interactions of HIV-1." AIDS Research and Human Retroviruses ja (2020).
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Kitakaze, Tomoya, et al. "Extracellular transglutaminase 2 induces myotube hypertrophy through G protein-coupled receptor 56." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1867.2 (2020): 118563.
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Dang-Nguyen, Thanh Quang, et al. "Combined refinements to somatic cell nuclear transfer methods improve porcine embryo development." Journal of Reproduction and Development (2020): 2019-156.
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Haraguchi, Seiki, et al. "Establishment of porcine nuclear transfer-derived embryonic stem cells using induced pluripotent stem cells as donor nuclei." Journal of Reproduction and Development (2020): 2019-137.
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Chen, Chen, et al. "Isolation of a novel bacterial strain capable of producing abundant extracellular membrane vesicles carrying a single major cargo protein and analysis of its transport mechanism." Frontiers in Microbiology 10 (2020): 3001.
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Noguchi, Taiki, et al. "Regulatory expression of bone morphogenetic protein 6 by 2, 2′-dipyridyl." Biochimica et Biophysica Acta (BBA)-General Subjects (2020): 129610.
Comparison of Price
|
Company
|
Nacalai USA, Inc.
|
Cytiva
|
|---|---|---|
|
Item
|
Chemi-Lumi One Ultra
|
Amersham ECL Select
Western Blotting Detection Reagent
|
|
Price
|
$328
|
$420
|
|
Cat. No.
|
11644-40
|
RPN2235
|
|
Quantity
|
for 1,000 cm² membrane
|
for 1,000 cm² membrane
|
Ordering Information
| Product | Storage | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| Chemi-Lumi One Ultra (Luminol 50 mL, Peroxide 50 mL, Sufficient substrate for 1,000 cm² of blotting membrane) | Room Temp. | 11644-40 | 1 Kit | 328.00 | Buy |
| Chemi-Lumi One Ultra (Luminol 10 mL, Peroxide 10 mL, Sufficient substrate for 200 cm² of blotting membrane) | Room Temp. | 11644-24 | 1 Kit | 116.00 | Buy |