Gel Filtration Chromatography (COSMOSIL Diol-II)
COSMOSIL Diol-120-II, Diol-300-II and Diol-1000-II gel filtration columns are ideal for the size-based separation of proteins and other water soluble polymers. The separation MW range is 5,000-700,000 daltons for proteins and 1000-500,000 daltons for water soluble polymers when Diol-120-II, Diol-300-II and Diol-1000-II are used in series.
Features
- Ideal for the size-based separation of proteins and water soluble polymers
- Reduce undesirable adsorption
| Packing Material | 5Diol-120-II | 5Diol-300-II | 5Diol-1000-II | 5Diol-2000-II |
|---|---|---|---|---|
| USP code | L20 | |||
| Silica Gel | High purity porous spherical silica * | |||
| Average Particle Size | 5 µm | |||
| Average Pore Size | approx. 120Å | approx. 300Å | approx. 1000Å | approx. 2000Å |
| Stationary Phase | Diol group | |||
| Suitable Samples | Protein, Water-Soluble Polymer | |||
| Flow Rate | 0.5-1.0 ml/min | |||
| Selection of Pore Size (Proteins) | 5,000-100,000 | 10,000-700,000 | - | - |
| Selection of Pore Size (Water Soluble Polymer) | 1,000-20,000 | 5,000-100,000 | 50,000-500,000 | - |
* With the silica-based gel, organic solvents, including methanol and acetonitrile, can be use.
Calibration Curve of Proteins
Condition
| Column: | COSMOSIL 5Diol-II 7.5mmI.D. x 600mm |
| Mobile phase: | 20mmol/l Phosphate Buffer (pH 7.0) +100mmol/l Na2SO4 |
| Flow rate: | 1.0ml/min |
| Temperature: | 30 °C |
| Sample | MW | Sample | MW | |
|---|---|---|---|---|
| Thyroglobulin | 660,000 | Peroxidase | 40,000 | |
| Catalase | 250,000 | Carbonic Anhydrase | 30,000 | |
| Glucose Oxidase | 160,000 | α -Chymotrypsinogen A | 25,700 | |
| Uricase | 128,000 | α -Chymotrypsin | 25,200 | |
| Choline Oxidase | 95,000 | Trypsinogen | 24,000 | |
| Transferrin | 85,000 | Trypsin (bovine) | 23,300 | |
| Conalbumin | 77,500 | Myoglobin | 17,000 | |
| Malate Dehydrogenase | 70,000 | Lysozyme | 14,300 | |
| α -Glucosidase | 68,500 | Ribonuclease A | 13,700 | |
| Albumin (BSA) | 66,000 | Cytochrome C | 12,400 | |
| α -Amylase | 52,500 | Aprotinin | 6,500 | |
| Fetuin | 48,000 | Gly-Gly | 132 | |
| Albumin (Ovalbumin) | 45,000 |
Comparison with Competitors' Columns
COSMOSIL Diol-II series shows excellent separation compared to competitors' columns.
| Column |
Column Size | Gel | Particle Size (avg.) | Pore Size (avg.) | Chart Speed |
|---|---|---|---|---|---|
| COSMOSIL 5Diol-300-II | 7.5 mm I.D. x 300 mm |
Silica Gel | 5 µm | approx. 300Å | 5 mm/min |
| TSKgel G3000 SWXL | 7.8 mm I.D. x 300 mm |
Silica Gel | 5 µm | 250Å | 5 mm/min |
| COSMOSIL 5Diol-120-II | 7.5 mm I.D. x 300 mm |
Silica Gel | 5 µm | approx. 120Å | 5 mm/min |
| TSKgel G2000 SWXL | 7.8 mm I.D. x 300 mm |
Silica Gel | 5 µm | 125Å | 5 mm/min |
COSMOSIL 5Diol-300-II
Condition
| Mobile phase: | 0.01mol/l Na2HPO4+0.01mol/l NaH2PO4+0.1mol/l Na2SO4 |
| Flow rate: | 1.0ml/min |
| Temperature: | 30°C |
| Detection: | UV220nm |
Sample
1. Ferritin (Horse)2. Peroxidase (Horse)
3. Cytochrome C (Horse)
4. Phenylglycine
COSMOSIL 5Diol-300-II
Condition
| Mobile phase: | 0.01mol/l Na2HPO4+0.01mol/l NaH2PO4+0.1mol/l Na2SO4 |
| Flow rate: | 1.0ml/min |
| Temperature: | 30°C |
| Detection: | UV220nm |
Sample
1. Transferrin (Human)2. Hemoglobin (Human)
3. Angiotensin II (Human)
COSMOSIL 5Diol-120-II
Condition
| Mobile phase: | 0.01mol/l Na2HPO4+0.01mol/l NaH2PO4+0.1mol/l Na2SO4 |
| Flow rate: | 1.0ml/min |
| Temperature: | 30°C |
| Detection: | UV220nm |
Sample
1. Sarcosine Dehydrogenase2. Lysozyme
3. Phenylglycine
COSMOSIL 5Diol-120-II
Condition
| Mobile phase: | 0.01mol/l Na2HPO4+0.01mol/l NaH2PO4+0.1mol/l Na2SO4 |
| Flow rate: | 1.0ml/min |
| Temperature: | 30°C |
| Detection: | UV220nm |
Sample
1. Sarcosine Dehydrogenase2. Lysozyme
3. Phenylglycine
Applications
E. Coli Proteins
Sodium Polystyrene Sulfonate
Polyethylene Glycols
Pullulan
Human Serum
Application: Polystyrene
COSMOSIL Application Search
COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size
Ordering Information
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL 5Diol-120-II Packed Column (4.6 mm I.D. x 150 mm) | End-Fitting: Waters type |
07490-51 | 1 pkg | 642.00 | Buy |
| COSMOSIL 5Diol-120-II Packed Column (4.6 mm I.D. x 250 mm) | End-Fitting: Waters type |
06888-91 | 1 pkg | 803.00 | Buy |
| COSMOSIL 5Diol-120-II Packed Column (7.5 mm I.D. x 300 mm) | End-Fitting: Waters type |
38050-51 | 1 pkg | 1,124.00 | Buy |
| COSMOSIL 5Diol-120-II Packed Column (7.5 mm I.D. x 600 mm) | End-Fitting: Waters type |
38051-41 | 1 pkg | 1,696.00 | Buy |
| COSMOSIL 5Diol-120-II Packed Column (10.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
12282-21 | 1 pkg | - | Inquire |
| COSMOSIL 5Diol-300-II Packed Column (4.6 mm I.D. x 150 mm) | End-Fitting: Waters type |
07223-51 | 1 pkg | 642.00 | Buy |
| COSMOSIL 5Diol-300-II Packed Column (4.6 mm I.D. x 250 mm) | End-Fitting: Waters type |
13086-71 | 1 pkg | 803.00 | Buy |
| COSMOSIL 5Diol-300-II Packed Column (7.5 mm I.D. x 300 mm) | End-Fitting: Waters type |
38053-21 | 1 pkg | 1,124.00 | Buy |
| COSMOSIL 5Diol-300-II Packed Column (7.5 mm I.D. x 600 mm) | End-Fitting: Waters type |
38054-11 | 1 pkg | 1,696.00 | Buy |
| COSMOSIL 5Diol-1000-II Packed Column (7.5 mm I.D. x 300 mm) | End-Fitting: Waters type |
13338-71 | 1 pkg | 2,140.00 | Buy |
| COSMOSIL 5Diol-2000-II Packed Column (7.5 mm I.D. x 300 mm) | End-Fitting: Waters type |
19976-31 | 1 pkg | 2,140.00 | Buy |
Guard Column / Guard Cartridge
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL 5Diol-120-II Guard Column (7.5 mm I.D. x 50 mm) | End-Fitting: Waters type |
38049-91 | 1 pkg | 530.00 | Buy |
| COSMOSIL 5Diol-300-II Guard Cartridge (4.6 mm I.D. x 10 mm) | End-Fitting: Waters type |
19180-54 | 2 pkg | 236.00 | Buy |
| COSMOSIL 5Diol-300-II Guard Column (7.5 mm I.D. x 50 mm) | End-Fitting: Waters type |
38052-31 | 1 pkg | 530.00 | Buy |
| COSMOSIL 5Diol-1000-II Guard Column (7.5 mm I.D. x 50 mm) | End-Fitting: Waters type |
13337-81 | 1 pkg | 535.00 | Buy |
HPLC Accessories
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) **Discontinued** | End-Fitting: Waters type |
38009-79 | 1 pkg | - | Inquire |
| COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) | End-Fitting: Waters type |
11884-71 | 1 pkg | 265.00 | Buy |
| COSMOSIL Column Prefilter | End-Fitting: Waters type |
39361-19 | 1 pkg | 145.00 | Buy |
| COSMOSIL Column Spare Filter for Prefilter (1 micron) | End-Fitting: Waters type |
39539-09 | 2 pkg | 55.00 | Buy |
| COSMOSIL Column Connecting Tube (0.25 mm) | End-Fitting: Waters type |
37843-69 | 1 pkg | 65.00 | Buy |
| COSMOSIL Column Connecting Tube (0.1 mm) | End-Fitting: Waters type |
12570-41 | 1 pkg | 65.00 | Buy |
#38009-79 has been discontinued. Alternative product is COSMOSIL Direct Cartridge Holder (#19989-71).