Hydrophilic interaction chromatography (HILIC) is a popular technique to analyze very polar molecules. However, it is often difficult to develop a robust method.



HILIC Columns: Disadvantages

・　Extremely sensitive to sample solvent used resulting in shifting retention time.

・　Small sample loading capacity – polar molecules do not dissolve well in organic solvents.

・　Peak distortion occurs if sample solvents are different than the mobile phase.



PBr Columns: Advantages

・　Retain very polar compounds in reversed-phase.

・　Use simple mobile phase, e.g. 100% aqueous or organic mobile phase.

・　Higher sample loading capacity than HILIC.

・　Robust

COSMOCORE (Core-shell based)

http://www.nacalaiusa.com/products/view/280/cosmocore-2-6pbr

COSMOSIL (Fully porus silica gel based)

http://www.nacalaiusa.com/products/view/238/cosmosil-5pbr